A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical ...molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time‐resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)33+ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single‐cell studies, the bio‐coreactant‐enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR‐1.
Amine‐rich biomolecules as consumed coreactants drive electrochemiluminescence with Ru(bpy)32+, enabling bio‐coreactant‐enhanced single‐cell electrochemiluminescence microscopy. This allows the imaging of intracellular hierarchical structures without the use of multiple labels. Dynamic signals disclose the universal edge effect of cellular electroporation and enable the visualization of heterogeneous molecular transport.
Antibacterial Carbon Dots‐Based Composites Huang, Shan; Song, Yuexin; Zhang, Jian‐Rong ...
Small (Weinheim an der Bergstrasse, Germany),
08/2023, Volume:
19, Issue:
31
Journal Article
Peer reviewed
Open access
The emergence and global spread of bacterial resistance to conventionally used antibiotics have highlighted the urgent need for new antimicrobial agents that might replace antibiotics. Currently, ...nanomaterials hold considerable promise as antimicrobial agents in anti‐inflammatory therapy. Due to their distinctive functional physicochemical characteristics and exceptional biocompatibility, carbon dots (CDs)‐based composites have attracted a lot of attention in the context of these antimicrobial nanomaterials. Here, a thorough assessment of current developments in the field of antimicrobial CDs‐based composites is provided, starting with a brief explanation of the general synthesis procedures, categorization, and physicochemical characteristics of CDs‐based composites. The many processes driving the antibacterial action of these composites are then thoroughly described, including physical destruction, oxidative stress, and the incorporation of antimicrobial agents. Finally, the obstacles that CDs‐based composites now suffer in combating infectious diseases are outlined and investigated, along with the potential applications of antimicrobial CDs‐based composites.
Carbon dots (CDs)‐based composites integrate the outstanding properties of functional modules and CDs, exhibiting remarkable optical, enzyme‐like activity, and biocompatible properties, emerging as a promising application tool in the field of bacteriostatic therapy.
Here, a sensitive and universal noncoding RNA (ncRNA) upconversion sensing nanoplatform is developed. Gold nanoparticles bearing one hairpin DNA (Hp) molecule are conjugated to the linker DNA ...modified NaYF4:Yb, Er@NaYF4 upconversion nanoparticles by DNA hybridization, leading to quenching of the upconversion emission through fluorescence resonance energy transfer. A signal DNA (SDNA) sequence is designed to open Hp, recovering the upconversion emission. To achieve universality and high sensitivity of the nanoprobe, an exonuclease III (Exo III)‐assisted cycling amplification strategy is introduced. A multifunctional hairpin DNA (mHp) containing ncRNA recognition sequence and SDNA sequence is designed to recognize ncRNA and trigger Exo III as a biocatalyst to stepwise disintegrate itself, releasing both ncRNA and SDNA. The released ncRNA can be reused to release more SDNA, which greatly improves the sensing sensitivity. By changing the recognition portion of mHp, various ncRNA can be detected. The sensitive detection of both homeobox (HOX) transcript antisense RNA segment and miR‐21 is achieved with this novel strategy, even in human serum, indicating the universality and sensitivity of the proposed strategy. Additionally, the expression level of miR‐21 in human breast cancer cell (MCF‐7) lysate is successfully measured, suggesting its potential in clinical diagnosis.
A universal and sensitive upconversion sensing nanoplatform is developed for sensitively detecting tumour‐related ncRNA by combining the upconversion nanoprobes with an exonuclease III‐assisted cycling amplification strategy. By changing the recognition portion of the substrate in cycling amplification, various ncRNA can be detected with high sensitivity and selectivity in complex biological samples, which has great potential for cancer diagnosis.
As a series of important metal materials, rare earth elements and their related derivative compounds have received significant attention because of their narrow emission bands, noncytotoxicity, ...excitation of multiple fluorescence colors and electrochemical properties. These unique properties endow rare earth-based nanomaterials with great potential for application in various fields such as in supercapacitors, batteries, sensors, devices and solar cells. This review presents a general description of the electrochemical properties of rare earth-based nanomaterials. The developments and improvements of their electrochemical applications in electrochemical sensors for the detection of small biomolecules and DNA, supercapacitors, batteries and electrochemical catalysis towards the hydrogen evolution reaction have also been discussed.
This review presents a general description of the synthesis and electrochemical properties of rare earth-based nanomaterials and their electrochemical applications.
Separators play a pivotal role in the electrochemical performance and safety of lithium‐ion batteries (LIBs). The commercial microporous polyolefin‐based separators often suffer from inferior ...electrolyte wettability, low thermal stability, and severe safety concerns. Herein, a novel kind of highly flexible and porous separator based on hydroxyapatite nanowires (HAP NWs) with excellent thermal stability, fire resistance, and superior electrolyte wettability is reported. A hierarchical cross‐linked network structure forms between HAP NWs and cellulose fibers (CFs) via hybridization, which endows the separator with high flexibility and robust mechanical strength. The high thermal stability of HAP NW networks enables the separator to preserve its structural integrity at temperatures as high as 700 °C, and the fire‐resistant property of HAP NWs ensures high safety of the battery. In particular, benefiting from its unique composition and highly porous structure, the as‐prepared HAP/CF separator exhibits near zero contact angle with the liquid electrolyte and high electrolyte uptake of 253%, indicating superior electrolyte wettability compared with the commercial polyolefin separator. The as‐prepared HAP/CF separator has unique advantages of superior electrolyte wettability, mechanical robustness, high thermal stability, and fire resistance, thus, is promising as a new kind of separator for advanced LIBs with enhanced performance and high safety.
A new kind of highly flexible, porous, high‐wettability, fire‐resistant hydroxyapatite nanowire‐based separator with superior performance and high safety is prepared for advanced lithium‐ion batteries. The batteries with the hydroxyapatite nanowire‐based separators show better cyclability and enhanced rate capability compared with those with the commercial polypropylene separator. The as‐prepared batteries adopting the hydroxyapatite nanowire‐based separator can safely work at 150 °C.
Risk analysis of investment decision-making behavior is to improve the ability to prevent investment risks in order to guarantee the success rate of investment decisions. In this paper, the risk ...evaluation level is determined by calculating the correlation degree of risk indicators through the establishment of the material topology matrix, and the gray evaluation coefficients are determined by using the gray correlation analysis of the whitening weight function to construct the gray evaluation model. The risk index factors and the overall gray evaluation were analyzed by using the material topology model and the gray evaluation model. From the physical topology evaluation, the market risk correlation is −0.00825, which means the special level risk. From the grey comprehensive evaluation score, the overall evaluation score is 6.884 overall risk level is medium grade line. This shows that using the model constructed in this paper can effectively realize the risk analysis of investment decisions and provide a guidance basis for investors to take effective measures for different types of risks.
A novel electrochemiluminescence (ECL) Janus emitter realizes dual‐mode biosensing for multiple disease‐related biomarkers from cells. Each Janus emitter consists of ruthenium (II) complex and ...luminol polymer doped hemispheres that are spatially separated but function simultaneously, generating intense dual‐ECL emissions. This design effectively eliminates optical and electrochemical interferences between different ECL mono‐luminophores in comparison with the homogenous nanostructures, thereby improving ECL efficiency and detection sensitivity. Accordingly, the ECL Janus emitters provide a proof of concept for the construction of the ratiometric ECL sensing of H2O2. Furthermore, this Janus emitter enables simultaneous identification and quantification of the intracellular cancer‐related dual microRNAs. This work not only inspires the development of ECL Janus emitters, but also highlights their potential in bioanalysis and clinical diagnosis in the future.
A novel Janus emitter consisting of ruthenium (II) complex and luminol polymers is synthesized and realizes dual‐electrochemiluminescence (ECL) without signal interferences. Accordingly, the ECL Janus emitters provides a proof of concept for the construction of ratiometric ECL sensing. Furthermore, the ECL Janus emitters enables simultaneous identification and quantification of intracellular disease‐related dual microRNAs.
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•The great potential of multifunctional nanomaterials for ECL bioanalysis is presented.•Deep insights into nanomaterials-based ECL biosensing strategies are discussed.•Recent ...significant developments of nanomaterials-based ECL biosensors are reviewed.•Novel applications of ECL resonance energy transfer and ECL imaging are highlighted.•Future challenges and prospects of nanomaterials-based ECL bioanalysis are concluded.
Nanomaterials with wonderful optical, electrical and chemical properties are widely studied in recent decades. In electrochemiluminescence (ECL) sensing area, nanomaterials have also gained crescent attention for their excellent performance based on different functions, such as innovative luminophores, molecule carriers, electrode modification materials and reaction catalysts. Deep insight into ECL biosensing strategies with multifunctional nanomaterials will benefit the design of advanced sensors. Based on this, nanomaterials-based ECL biosensors have shown more promising potentials than traditional ones in analytical applications. After a brief overview of basic ECL principles and sensing approaches, herein, a general description of nanomaterials-based ECL biosensing is presented, especially with emphasis on recently developed ECL resonance energy transfer (ECL-RET) strategy. Finally, future outlooks are considered in building sensitive ECL biosensors.
Tin (Sn) is known to be a good catalyst for electrochemical reduction of CO2 to formate in 0.5 M KHCO3. But when a thin layer of SnO2 is coated over Cu nanoparticles, the reduction becomes ...Sn-thickness dependent: the thicker (1.8 nm) shell shows Sn-like activity to generate formate whereas the thinner (0.8 nm) shell is selective to the formation of CO with the conversion Faradaic efficiency (FE) reaching 93% at −0.7 V (vs reversible hydrogen electrode (RHE)). Theoretical calculations suggest that the 0.8 nm SnO2 shell likely alloys with trace of Cu, causing the SnO2 lattice to be uniaxially compressed and favors the production of CO over formate. The report demonstrates a new strategy to tune NP catalyst selectivity for the electrochemical reduction of CO2 via the tunable core/shell structure.
