Asthma is a chronic respiratory disorder of airways characterized by inflammation, hyperresponsiveness, inflammatory cell infiltration, mucous secretion, and remodelling. Ammi majus is medicinal ...plant belong to family of Apiaceous which has anti-inflammatory and antioxidant activities. This study designed to investigate of anti-asthmatic activity of alcoholic extract of Ammi majus in improvement of asthma. Forty-eight healthy female mice divided to six groups Group I: the negative control group (distal water only), Group II: Positive control group (ovalbumin group), Group III: Ammi majus (64 mg/kg/day) with sensitization, Group IV:Ammi majus (128 mg/kg/day) with sensitization, Group V: Ammi majus (64 mg/kg/day) without sensitization, Group VI: Ammi majus (128mg/kg/day) without sensitization. Mice were sacrificed by diethyl ether and blood samples were collected to prepare of serum samples that used in ELISA kits for measuring of parameter IL-4, IL-5, IL-33, & IgE. Levels of all parameters (IL-4, IL-5, IL-33, & IgE) for mice of treated groups with alcoholic extract of Ammi majus were highly significant reduced (p<0.05) in compared to ovalbumin group.in conclusion, our results demonstrated that alcoholic extract of Ammi majus has a potent anti asthmatic activity that improved ovalbumin-induced asthma.
Twenty target compounds were designed, synthesized and evaluated in terms of their biological activity.
Display omitted
Twenty benzothiazole derivatives bearing a 1,3,4-oxadiazole moiety were ...synthesized and evaluated for their anti-oxidant and anti-inflammatory activities. Among these compounds, 8h and 8l were appeared to have high radical scavenging efficacies as 0.05 ± 0.02 and 0.07 ± 0.03 mmol/L of IC50 values in ABTS+ bioassay, respectively. In anti-inflammatory tests, compound 8h displayed good activity with 57.35% inhibition after intraperitoneal administration, which was more potent than the reference drug (indomethacin). Molecular modeling studies were performed to investigate the binding mode of the representative compound 8h into COX-2 enzyme. In vitro enzyme study implied that compound 8h exerted its anti-inflammatory activity through COX-2 inhibition.
There are few studies on seaweed polysaccharides with UV/H2O2 treatment, so the aim of this study was to evaluate the effects of UV/H2O2 treatment on physicochemical properties and RAW 264.7 cells ...responses of polysaccharides from Sargassum fusiforme (PSF). Results showed that the contents of reducing sugar and sulfate in PSF with UV/H2O2 treatment for 2 h increased by 202.86% and 31.77%, respectively, and the contents of total sugar, protein and uronic acid decreased by 14.29%, 57.11% and 43.18% compared with those of original polysaccharides. In addition, UV/H2O2 treatment did not change the monosaccharide types of original polysaccharides, but it could change its monosaccharide composition and surface morphology. Besides, polysaccharides after UV/H2O2 treatment for 0.5–2 h had lower toxicity than original polysaccharides in RAW 264.7 cells. Typically, PSF with UV/H2O2 treatment for 2 h (PSF-T2) could effectively inhibit pro-inflammatory molecules production (including NO, IL-1β, IL-6 and TNF-α), and down-regulate related genes expression (including Tlr4, Irak, Il-1β, Il-6, Il-12 and Tnf-α). Therefore, UV/H2O2 treatment is a potential way to prepare polysaccharide with better anti-inflammatory activity.
Display omitted
•UV/H2O2 treatment changed the physicochemical properties of PSF.•PSF after UV/H2O2 treatment had lower toxicity in RAW 264.7 cells.•PSF after UV/H2O2 treatment for 2 h had a good anti-inflammatory activity.
Bio‐based products are nowadays useful tools able to affect the productivity and quality of conventionally cultivated crops. Several bio‐based products are currently on the market; one of the newest ...and most promising is the wood distillate (WD) derived from the pyrolysis process of waste biomass after timber. Its foliar application has been widely investigated and shown to promote the antioxidant profile of cultivated crops. WD was used here as additive for the cultivation of tomato (Solanum lycopersicum L.) plants. The application improved quality (chemical) parameters, minerals, polyphenols, and lycopene contents of tomato fruits. The extracts of WD‐treated and untreated tomatoes have been chemically and biologically characterized. The 1H‐NMR and ESI‐mass spectrometry analyses of the extracts revealed the presence of different fatty acids, amino acids and sugars. In particular, the WD‐treated tomatoes showed the presence of pyroglutamic acid and phloridzin derivatives, but also dihydrokaempferol, naringenin glucoside, cinnamic acid, and kaempferol‐3‐O‐glucoside. When tested in cells, the extracts showed a promising anti‐inflammatory profile in lipopolysaccharide (LPS)‐stimulated RAW 264.7 macrophages. Furthermore, the extracts displayed a slight vasorelaxant activity on rat aorta rings (either endothelium‐denuded or endothelium‐intact) pre‐contracted with phenylephrine or potassium chloride.
Practical Application
Wood distillate has been used for tomato plant growth. Tomatoes showed improved nutritional parameters, and their extracts displayed antioxidant and anti‐inflammatory activities.
Lipopolysaccharides from Bacteroides vulgatus represent interesting targets for the treatment of inflammatory bowel diseases. In their Research Article (e202301351), Guozhi Xiao and co‐workers ...accomplished the first synthesis of a tridecasaccharide motif from Bacteroides vulgatus lipopolysaccharides by a strategy involving orthogonal one‐pot glycosylation reactions based on glycosyl ortho‐(1‐phenylvinyl)benzoates, which avoided issues such as aglycone transfer and the unpleasant odor of thioglycoside‐based orthogonal one‐pot glycosylation.
The nutraceutical value, and physicochemical profile as well as anti-inflammatory activity potential of Odonthalia floccose and Odonthalia dentata (red macroalgae) dry biomass were investigated in ...this study. Proximate composition study results revealed that the dry biomass of O. floccose and O. dentae were found to be as ash: 9.11 & 8.7 g 100 g-1, moisture: 8.24 & 8.1 g 100 g-1, total fat: 6.9 & 7.2 g 100 g-1, protein: 24.52 & 25.6 g 100 g-1, and total carbohydrate/polysaccharides: 53.84 & 48.85 g 100 g-1 of dry weight biomass respectively. Both algae biomass contain considerable quantity of minerals (Fe, Cu, Mg, and Zn). Furthermore, the major saturated fatty acids (6.24 & 5.82 g FAME 100 g-1 of total fat of O. floccose and O. dentate) (ΣFAs) present in the test algae were stearic acid, palmitic acid, and margaric acids. O. floccose and O. dentata also contain remarkable protein composition profile that compiled with considerable quantity of essential and non-essential amino acids. The vitamins such as vitamin A, B1, B2, B3, B6, B9, C, and E of O. floccose and O. dentate biomass were also identified at sufficient quantity level. The swelling capacity (SWC), water holding capacity (WHC), and oil holding capacity (OHC) properties of O. floccose and O. dentate at various temperature conditions (25 and 37 ᵒC) were found to be 8.11 & 7.02 mL g-1 and 8.95 & 7.55 mL g-1, 5.1 & 4.87 and 4.8 & 4.1 mL g-1, as well as 2.11 & 1.81 and 1.96 & 1.89 mL g-1 respectively. Among these two marine red macroalgae samples, the O. dentate showed better anti-inflammatory activity than O. floccose at 150 μg mL-1 dosage. Thus, this O. floccose and O. dentate biomass can be considerable as nutritional supplement and pharmaceutical product development related research.
•O.floccose and O. dentae possesses fine proximate parameters•Both these test red algae contain considerable quantity of minerals•Stearic acid, palmitic acid, & margaric acids found as major saturated fatty acids•Vitamin A, B, C, & E well available in O. floccose and O. dentate biomass•O. dentate showed better anti-inflammatory activity than O. floccose at 150μg mL-1
Aquaculture is one of the fastest growing sectors in global food production, recognized as a significant contributor to poverty alleviation, food security, and income generation. However, the ...frequent occurrence of diseases caused by pathogen infections result in reduced yields and economic losses, posing a substantial constraint to the sustainable development of aquaculture. Here, our study identified that four catechol compounds, quercetin, luteolin, caffeic acid, and chlorogenic acid, exhibited potent antiparasitic effects against Ichthyophthirius multifiliis in both, in vitro and in vivo. The parasite is recognized as one of the most pathogenic to fish worldwide. Using a combination of in silico methods, the dipeptidyl peptidase (DPP) was identified as a critical target for catechol compounds. The two hydroxyl radicals of the catechol group were essential for its binding to and interacting with the DPP protein. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that catechol compounds disrupt pathways associated with the metabolism and growth of I. multifiliis, thereby exerting antiparasitic effects. Furthermore, these compounds attenuated the expression of proinflammatory cytokines in vivo in fish and promoted macrophage polarization toward M2 phenotype by inhibiting the STAT1 signaling pathway. The dual activity of catechol compounds, acting as both direct antiparasitic and anti-inflammatory agents in fish, offers a promising therapeutic approach for combating I. multifiliis infections in aquaculture.
Display omitted
•We proposed an effective dual-targeting mechanism for the protection of aquatic animal against I. multifiliis infections.•A combined in silico strategy was employed to identify the molecular target and elucidate the mechanism.•The catechol group was identified as a pharmacophore with dual effects, exhibiting both anti-inflammatory and antiparasitic activity.
Display omitted
•PLLA/CS micro/nano-fibrous scaffold was prepared by 3D printing and TIPS method.•3D CS nanofibers favor cell growth and adhesion into the interior of the scaffold.•Qu, a drug, was ...first fixed on the scaffold surface and released continuously.•The PLLA/CS-D/Qu scaffold has excellent osteogenic and anti-inflammatory activity.•The as-prepared PLLA/CS-D/Qu scaffold has potential as a bone repair material.
We present here a porous poly (L-lactide)/chitosan (PLLA/CS) composite scaffold with micro/nano-fiber hierarchical structure that was innovatively fabricated by combining 3D printing and thermally induced phase separation technology. Moreover, bioactive quercetin (Qu) was chosen to further functionalize the PLLA/CS scaffold with polydopamine (PDA) layer. The final results showed that the hydrophilicity and mechanical properties of the resulting PLLA/CS-D/Qu scaffold were obviously superior to those of the pure 3D printing PLLA scaffold. In vitro MC3T3-E1 cell experiments suggested that the introducing CS nanofiber network helped cells grow into the interior of the scaffold and promoted cell adhesion and proliferation. More importantly, the synergistic effect of the CS nanofiber network, PDA layer and especially sustain-released Qu, can endow the PLLA/CS-D/Qu scaffolds with not only excellent cell affinity and osteogenic activity, but also good anti-inflammatory property. Thus, the PLLA/CS-D/Qu scaffolds are expected to have good application prospects in the field of bone tissue engineering.
In the present study, crude polysaccharides were extracted from Gelidium pacificum Okamura, and further purified to obtain the sulfated polysaccharide with molecular weight of 28,807 Da. Its ...monosaccharide composition mainly consisted of xylose (7.1%), galactose (59.7%) and galacturonic acid (19.76%). And the sulfate ester content of the sulfated polysaccharide was estimated as 8.8%. Structure analysis showed that the sulfated polysaccharide comprised of 1,4-linked-α-D-Galp3S, 1,2-linked-α-D-Xylp and 1,3-linked-β-D-GalpA residues, respectively. Its anti-inflammatory effects were investigated in LPS-stimulated human monocytic (THP-1) cells. The sulfated polysaccharide at a concentration of 5 μg/mL fully protected the THP-1 cells against LPS-stimulated cytotoxicity. Furthermore, the addition of sulfated polysaccharide resulted in a significant reduction of NO production in LPS-treated cells, and this effect appeared to be dose-related. The sulfated polysaccharide (5 μg/mL) significantly suppressed the mRNA and protein expression of toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumor necrosis factor receptor-associated factor-6 (TRAF-6) in LPS-stimulated THP-1 cells. These results showed the sulfated polysaccharide not only provided a good protection against LPS-induced cell toxicity, but also exerted an anti-inflammatory effect via the TLR4 signaling pathway.
•The isolation and purification of Gelidium pacificum Okamura polysaccharide (sulfated polysaccharide) were first studied.•The structure of the sulfated polysaccharide was analyzed and identified.•The sulfated polysaccharide exhibited a protective effect against LPS-stimulated cell toxicity.•The anti-inflammatory mechanisms of the sulfated polysaccharide were studied.
