A Gram-negative, rod-shaped and aerobic bacterial strain B3.7 T , was isolated from the sediment of Zhairuo Island, Zhoushan city, Zhejiang Province, PR China. Maximum growth of strain B3.7 T was ...observed at 30 °C when cultured in a medium containing 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain B3.7 T belonged to the genus Shinella ; it showed the highest sequence similarity of 98.47 % to Shinella kummerowiae CCBAU 25048 T . The average nucleotide identity and digital DNA–DNA hybridization values between strain B3.7 T and its reference strains were 82.9–84.2 % and 26.1–27.3 %, respectively. Chemotaxonomic analysis indicated that the sole respiratory quinone was Q-10 and the predominant cellular fatty acids were C 19 : 0 cyclo ω 8 c , C 16 : 0 , C 18 : 1 ω 7 c 11-methyl and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ). The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and two unidentified aminolipids. Collectively, strain B3.7 T can be considered to represent a novel species, for which the name Shinella sedimenti sp. nov. is proposed. The type strain is B3.7 T (=MCCC 1K07163 T =LMG 32559 T ).
A Gram-reaction-negative, strictly aerobic, pale yellow, non-gliding, rod-shaped bacterium, designated DT-LB-19 T , was isolated from the sediment of East Taihu Lake in Jiangsu Province, PR China. ...Strain DT-LB-19 T showed the highest 16S rRNA gene sequence similarities to members of the genera Algoriella , Chishuiella and Empedobacter (94.84–95.77 %) in the family Weeksellaceae . In phylogenetic trees based on genomes, strain DT-LB-19 T clustered within the genus Empedobacter but formed a separate subclade with a high bootstrap value. The average nucleotide identity and digital DNA–DNA hybridization values between DT-LB-19 T and the closely related type strains were in the range of 82.5–86.9 % and 25.8–32.3 %, respectively. The major cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH, C 16 : 1 ω 5 c , C 16 : 0 , summed feature 4 (iso-C 17 : 1 I and/or anteiso-C 17 : 1 B), summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and iso-C 15 : 0 3-OH. The predominant menaquinone was menaquinone-6. The polar lipid profile consisted of phosphatidylethanolamine, one glycolipid, two aminophospholipids and five unidentified lipids. The DNA G+C content was 31.8 mol%. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic results, we propose that strain DT-LB-19 T represents a novel species of the genus Empedobacter , for which the name Empedobacter sedimenti sp. nov. is proposed, with strain DT-LB-19 T (=KCTC 82330 T =CCTCC AB 2023026 T = JSACC 11448 T ) as the type strain.
A Gram-stain-negative, rod and rod-curved shaped motile bacterium designated strain S25
was obtained from benthic sediment collected near the Kubbar Island coral reefs south of Kuwait. Phenotypic ...analysis revealed that strain S25
was slightly halophilic, mesophilic and facultative anaerobic, fermenting d-glucose, d-ribose, d-mannose, d-mannitol, maltose, fructose, gentiobiose, cellobiose, melibiose, trehalose and sucrose. It was positive for oxidase and indole production and negative for arginine dihydrolase and lysine and ornithine decarboxylases. It contained C
7
/C
6
(summed feature 3), C
ω7
(summed feature 8) and C
as the major fatty acids. Strain S25
grew optimally at 30 °C and pH 8 in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA sequences revealed that strain S25
is related to species of the genus
, having 99.15 % similarity to '
' AK16
, 99.08 % to
96-237
and 98.66 % to
IMCC 5001
. The DNA G+C content was 48.8 mol% and the full genome analysis for the strain S25
showed that the bacterium has a genome size of 5 158 621 bp and contains 4730 predicted protein-encoding genes. The average nucleotide identity values between the S25
genome and the genomes of its nearest matches ranged between 81.39 and 94.16 %. The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties. On the basis of the phenotypic, phylogenetic and genetic data, strain S25
represents a novel species in the genus
, for which the name
sp. nov. is proposed. The type strain of
is S25
(=DSM 28878
=LMG 28315
).
In this study, two novel alkalitolerant strains (FJAT-53046 T and FJAT-53715 T ) were isolated from sediment samples collected in Zhangzhou, PR China. Phylogeny based on 16S rRNA gene sequences ...suggested that strains FJAT-53046 T and FJAT-53715 T were new members of the genus Pseudalkalibacillus . The two novel strains showed the highest 16S rRNA gene sequence similarity to Pseudalkalibacillus hwajinpoensis DSM 16206 T , with values of 97.4 and 97.6 %, respectively. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two strains and the reference strain were 77.2 and 79.6 %, 20.9 and 30.2 %, respectively, which were below the prokaryotic species delineation thresholds. The ANI and dDDH values between strains FJAT-53046 T and FJAT-53715 T were 86.0 and 30.2 %, respectively, suggesting that they belonged to different species in the genus Pseudalkalibacillus . The major respiratory quinone in both strains was MK-7 and the major cellular fatty acids were anteiso-C 15 : 0 and anteiso-C 17 : 0 . Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids in both novel strains. Combined with results stemming from the determination of physical and biochemical characteristics, chemical properties, and genome analysis, strains FJAT-53046 T and FJAT-53715 T are proposed to represent two novel species of the genus Pseudalkalibacillus , for which the names Pseudalkalibacillus spartinae sp. nov. and Pseudalkalibacillus sedimenti sp. nov. are proposed. The type strains are FJAT-53046 T (=GDMCC 1.3077 T =JCM 35611 T ) and FJAT-53715 T (=GDMCC 1.3076 T =JCM 35610 T ), respectively.
A Gram-stain-negative bacterium, designated XZ-24
, was isolated from sediment of a river in Mianyang city, Sichuan province, PR China. Cells (1.0-2.0 µm long and 0.4-0.5 µm in width) were strictly ...aerobic, non-spore-forming, rod shaped, prosthecate and motile by means of a polar flagellum. Growth occurred at 10-37 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum pH 7.0) and with 0-3.0 % (w/v) NaCl (optimum 1.0 % NaCl). The results of phylogenetic analysis based on genomes and 16S rRNA gene sequences indicated that XZ-24
formed a distinct phyletic branch within the family
and was most closely related to members of the genera
,
and
with 95.3-96.5 % 16S rRNA gene sequence similarities. The average amino acid identities (AAI) between XZ-24
and species of the family
were 47.0-64.5 %, which were below the genus boundary (70 %). The predominant cellular fatty acids were summed feature 8 (C
ω7
and/or C
ω6
), C
, C
ω7
11-methyl and summed feature 3 (C
ω7
and/or C
ω6
), the isoprenoid quinone was Q-10, and the major polar lipids were 1,2-di-O-acyl-3-O-α-d-glucopyranuronosyl glycerol; 1,2-di-O-acyl-3-O-d-glucopyranosyl-(1→4)-α-d glucopyranuronosyl glycerol and phosphatidylglycerol. The genome size of XZ-24
was 2.64 Mb with a DNA G+C content of 68.9 %. On the basis of the evidence presented in this study, strain XZ-24
represents a novel species of a novel genus in the family
, for which the name
gen. nov., sp. nov. (Type strain XZ-24
=CCTCC AB 20 23 094
=KCTC 8038
) is proposed.
A Gram-stain-negative bacterium, designated LG-4
, was isolated from sediment of Qiantang River in Zhejiang Province, PR China. Cells were strictly aerobic, non-spore-forming, non-motile and ...short-rod-shaped (1.0-1.2 µm long and 0.7-0.8 µm wide). Growth occurred at 15-42 °C (optimum, 30 °C), at pH 5.0-9.0 (pH 7.0) and at 0-2.0 % (w/v) NaCl (optimum, 0.5 % NaCl). Strain LG-4
showed 95.75-96.90 % 16S rRNA gene sequence similarity to various type strains of the genera
,
,
,
and
of the family
, and the most closely related strain was
ZQBW
(96.90 % similarity). The phylogenomic tree showed that strain LG-4
clustered in the family
and was positioned outside of the clade composed of the genera
and
. The average nucleotide identity and digital DNA-DNA hybridization values between strain LG-4
and the related type strains were in the range of 74.19-77.56 % and 16.70-25.80 %, respectively. The average amino acid identity (AAI) values between strain LG-4
and related type strains of the family
were 60.94-69.73 %, which are below the genus boundary (70 %). The evolutionary distance (ED) values between LG-4
and the related genera of the family
were 0.21-0.34, which are within the recommended standard (≥0.21-0.23) for defining a novel genus in the family
. The predominant cellular fatty acids were C
ω7
, C
cyclo ω8
, C
and C
, the isoprenoid quinone was Q-10, and the major polar lipids were phospholipid, phosphatidylglycerol, phosphatidylcholine, aminolipid and two unknown polar lipids. The genome size was 4.7 Mb with 68.6 mol% G+C content. On the basis of distinct phylogenetic relationships, low AAI values and high ED values, and differential phenotypic, physiological and biochemical characteristics, strain LG-4
represents a novel species of a new genus in the family
, for which the name
gen. nov., sp. nov. is proposed. The type strain is LG-4
(=MCCC 1K08849
=KCTC 8136
).
A Gram-stain-negative, motile (by single polar flagellum) and rod-shaped bacterium, designated W1-6
T
, was isolated from a sediment of drainage ditch in winery in Guiyang, south-western China. ...Strain W1-6
T
showed the highest 16S rRNA gene sequence similarities with the type strain of
Acidovorax wautersii
(98.1%) and
Simplicispira lacusdiani
(97.9%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W1-6
T
was placed adjacent to the members of the genus
Simplicispira
and formed a separat subclade. Cells showed oxidase and catalase negative reactions. The only respiratory quinone detected was ubiquinone-8 (Q-8). Summed feature 3 (C
16:1
ω
7
c
and/or C
16:1
ω
6
c
), C
16:0
and summed feature 8 (C
18:1
ω
7
c
and/or C
18:1
ω
6
c
) were predominant cellular fatty acids (> 10%) of strain W1-6
T
. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and five unidentified phospholipids were found in the polar lipid extraction. The genomic DNA G + C content was 65.6%. Strain W1-6
T
shared the highest digital DNA-DNA hybridization dDDH, (27.6%) and average nucleotide identity ANI (84.3%) values with the type strain of
S. lacusdiani.
The dDDH and ANI values were below the cutoff level (dDDH 70%; ANI 95–96%) for species delineation. The polyphasic characteristics indicated that the strain W1-6
T
represents a novel species of the genus
Simplicispira
, for which the name
Simplicispira sedimenti
sp. nov. is proposed. The type strain is W1-6
T
(= CGMCC 1.16274
T
= NBRC 115624
T
).
A novel bacterium, designated CAU 1612
, was isolated from coastal sediment sampled in the Republic of Korea. Cells of the strain were Gram-stain-negative, rod-shaped and motile. Optimum growth ...occurred at 30 °C, at pH 7.0 and with 1.0 % (w/v) NaCl. Based on 16S rRNA gene sequences, phylogenetic analysis revealed that strain CAU 1612
belonged to the genus
and showed highest similarity to '
' JCM 33810
(98.5 %), followed by
KCTC 32476
(97.7 %),
KCTC 12842
(96.0 %),
LMG 23452
(95.5 %),
KCTC 52846
(95.5 %) and
KCTC 12842
(95.4 %). The average nucleotide identity and digital DNA-DNA hybridization values of strain CAU 1612
were estimated to be 68.5-81.7 % and ≤23.7 %, respectively, to members of the genus
. The DNA G+C content was 54.1 mol%. The major cellular fatty acids (>10 %) were C
, C
cyclo
8
and summed feature 8 (C
7
and/or C
6
). The major menaquinone was Q-10 and the predominant polar lipids were phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. On the basis of taxonomic evidence, strain CAU 1612
represents a novel species, for which the name
sp. nov. is proposed. The type strain is CAU 1612
(=KCTC 82427
=MCCC 1K06082
).
A Gram-negative, strictly aerobic, non-motile, rod-shaped bacterial strain CAU 1614
T
was isolated from a marine sediment sample collected in the Republic of Korea. Optimal growth of strain CAU 1614
...T
proceeded at 30 °C, pH 7.0, and 2% (w/v) NaCl. 16S rRNA gene similarity was lower than 94.5% with genera
Aureisphaera
,
Marinirhabdus
,
Aureitalea
,
Gilvibacter
,
Ulvibacter
, and
Jejudonia
. The highest similarity was with
Aureisphaera galaxeae
04OKA003-7
T
(94.5%). The major cellular fatty acids were iso-C
15:0
, iso-C
16:0
, iso-C
15:1
G, iso-C
16:0
3-OH, and iso-C
17:0
3-OH and the predominant menaquinone was MK-6. The polar lipids were phosphatidylethanolamine, phosphoglycolipid, an unidentified lipid, two unidentified aminolipids, and an unidentified glycolipid. The draft genome of strain CAU 1614
T
was 3.9 Mb and DNA G+C content was 36.0 mol%. On the basis of the phenotypic, chemotaxonomic, and genomic data, strain CAU 1614
T
presents a novel genus in the family
Flavobacteriaceae
, for which the name
Halomarinibacterium sedimenti
gen. nov., sp. nov. is proposed. The type strain is CAU 1614
T
(= KCTC 82457
T
= MCCC 1K06083
T
).
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