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  • Investigating the specificity of endothelin-traps as a potential therapeutic tool for endothelin-1 related disorders [Elektronski vir]
    Jain, Arjun ...
    BACKGROUND: Endothelin (ET)-traps are Fc-fusion proteins with a design based on the physiological receptors of ET-1. Previous work has shown that use of the selected ET-traps potently and ... significantly reduces different markers of diabetes pathology back to normal, non-disease levels. AIM To demonstrate the selected ET-traps potently and significantly bind to ET-1. METHODS: We performed phage display experiments to test different constructs of ET-traps, and conducted bio-layer interferometry binding assays to verify that the selected ET-traps bind specifically to ET-1 and display binding affinity in the double-digit picomolar range (an average of 73.8 rM, n = 6). RESULTS: These experiments have confirmed our choice of the final ET-traps and provided proof-of-concept for the potential use of constructs as effective biologics for diseases associated with pathologically elevated ET-1. CONCLUSION: There is increased need for such therapeutics as they could help save millions of lives around the world.
    Source: World journal of diabetes [Elektronski vir]. - ISSN 1948-9358 (Vol. 13, iss. 6, 2022, str. 434-441)
    Type of material - e-article ; adult, serious
    Publish date - 2022
    Language - english
    COBISS.SI-ID - 110850819

    Link(s):

    https://f6publishing.blob.core.windows.net/d6f4e4d0-97f2-4e1a-a280-67aeee1b929d/WJD-13-434.pdf
    https://www.wjgnet.com/1948-9358/abstract/v13/i6/434.htm
    DOI

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