Transformacija tobaka (Nicotiana tobacum) cv. Samsun in krompirja (Solanum tuberosum L.) cv. Igor : diplomska naloga = Transformation of tobacco (Nicotiana tobacum) cv. Samsun and potato (Solanum tuberosum L.) cv. Igor : graduation thesis
Štrucl, Renate
Potato tuber necrotic ringspot disease, caused by necrotic strain of potato virus PVYNTN, is one of the most harmful diseases that infected Slovenian potato crops in the last century. In order to ...obtain successful system for thepotato Solanum tuberosum cv. Igor and to develop virus-resistant transgenicplants, the transformation plant technique with insertion of gene was used. The capability of regeneration and calli formation on potato plant Solanum tuberosum cv. Igor was observed on the media with different concentration of the kanamycin. The results of the study concluded that shoot growth is the least obstructive with 50% decreased concentration of kanamycin.The tobacco plants Nicotiana tabacum cv. Samsung and the potato plants Solanum tuberosum cv. Igor were infected with Agrobacterium tumefaciens. Therefore, a gene block with coat protein (CP) gene of virus PVYNTN - in sense orientation or gene which coded unfunctional protein and also a gene for kanamycin selection were introduced in Agrobacterium tumefaciens strain. During the process of Nicotiana tabacum transformation, anincreased virus-resistance and stable transgenic lines were developed, whichhas been preserved in tissue culture with micropropagation technique and was afterward transferred to the soil in a greenhouse. The highest ratio of calli formation, the highest number of shoots and the highest transformation was achieved on internodie explants. Furthermore, the transformation of potatoSolanum tuberosum cv. Igor obtained more stabile transformed plants as well, which were then used for further tests of resistance against PVYNTN virus.
Type of material - undergraduate thesis
Publication and manufacture - Ljubljana : Biotehniška fakulteta, Oddelek za biologijo, 2000
National Institute of Biology and BF, Department of Biology, Ljubljana
BF oddelek za biologijo dn 581.1 ŠTRUCL R. Transformacija IN: 0030301
available - reading room
Shelf entry
Adding material to shelf was successful.
Adding material to shelf failed.
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
