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Impact of deoxynivalenol and zearalenone as single and combined treatment on DNA, cell cycle and cell proliferation in HepG2 cells [Elektronski vir]Domijan, Ana-Marija ...The study aimed to investigate toxicity and the mechanism of toxicity of two Fusarium mycotoxins, deoxynivalenol (DON) and zearalenone (ZEA). DON and ZEA were applied to HepG2 cells as single ... compounds and in combination at low environmentally relevant concentrations. HepG2 cells were exposed to DON (0.5, 1, and 2 µM), ZEA (5, 10, and 20 µM) or their combinations (1 µM DON + 5 µM ZEA, 1 µM DON + 10 µM ZEA and 1 µM DON + 20 µM ZEA) for 24 h and cell viability, DNA damage, cell cycle and proliferation were assessed. Both mycotoxins reduced cell viability, however, combined treatment with DON and ZEA resulted in higher reduction of cell viability. DON (1 µM) induced primary DNA damage, while DON (1 µM) in combination with higher ZEA concentrations showed antagonistic effects compared to DON alone at 1 µM. DON arrested HepG2 cells in G2 phase and significantly inhibited cell proliferation, while ZEA had no significant effect on cell cycle. The combined treatment with DON and ZEA arrested cells in G2 phase to a higher extend compared to treatment with single mycotoxins. Potentiating effect observed after DON and ZEA co-exposure at environmentally relevant concentrations indicates that in risk assessment and setting governments’ regulations, mixtures of mycotoxins should be considered.Source: International journal of molecular sciences [Elektronski vir]. - ISSN 1422-0067 (Vol. 24, iss. 4, Feb. 2023, str. [1]-13)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 142398979
Author
Domijan, Ana-Marija |
Hercog, Klara |
Štampar, Martina, 1991- |
Gajski, Goran |
Gerić, Marko |
Sokolović, Marijana |
Žegura, Bojana
Topics
mikotoksini |
deoxynivalenol |
zearalenone |
citotoksičnost |
genotoksičnost |
celični cikel |
mycotoxins |
comet assay |
flow cytometry |
co-exposure |
food monitoring
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Author | Domijan, Ana-Marija ... |
Title | Impact of deoxynivalenol and zearalenone as single and combined treatment on DNA, cell cycle and cell proliferation in HepG2 cells [Elektronski vir] |
Publication date | 2023-02-17 |
COBISS.SI-ID | 142398979 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
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Ekotoksiologija, toksikološka genomika in karcinogeneza | P1-0245-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Domijan, Ana-Marija | ![]() |
Hercog, Klara | 36321 |
Štampar, Martina, 1991- | 39119 |
Gajski, Goran | ![]() |
Gerić, Marko | ![]() |
Sokolović, Marijana | ![]() |
Žegura, Bojana | 20767 |
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