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β-hydroxysteroid dehydrogenases types 1 and 2 : enzymatic assays based on radiometric and mass-spectrometric detectionSinreih, Maša ...The 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1) has a key role in estrogen biosynthesis as it catalyzes the reduction of estrone to the most potent estrogen, estradiol. Estradiol has a high ... affinity for estrogen receptors and thus stimulates their transactivation, which leads to cell proliferation and numerous other effects. HSD17B2 catalyzes the oxidation of estradiol to the less potent estrone, thereby decreasing estrogen receptor activation, which results in reduction of estrogen-associated effects. HSD17B1 and HSD17B2 overexpressing E. coli homogenates or recombinant enzymes can be used for screening and development of drugs against various pathologies such as cancer, endometriosis or osteoporosis. Here we describe the preparation of HSD17B1 and HSD17B2 bacterial homogenates and purified recombinant HSD17B1 protein as enzyme sources as well as enzymatic assays based on radiometric and mass-spectrometric detection for enzyme characterization.Source: Methods in enzymology. - ISSN 0076-6879 (Vol. 689, 2023, str. 201-234)Type of material - article, component partPublish date - 2023Language - englishCOBISS.SI-ID - 151647491
Author
Sinreih, Maša |
Gjorgoska, Marija |
Möller, Gabriele |
Adamski, Jerzy, molekularna biologija |
Lanišnik-Rižner, Tea
Topics
HSD17B1 |
HSD17B2 |
prekomerno izražanje Coli |
kinetična karakterizacija |
rekombinantni protein |
HSD17B1 |
HSD17B2 |
Coli overexpression |
kinetic characterization |
recombinant protein
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Sinreih, Maša | 34259 |
Gjorgoska, Marija | 55074 |
Möller, Gabriele | |
Adamski, Jerzy, molekularna biologija | 55378 |
Lanišnik-Rižner, Tea | 11699 |
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