Spremljanje stabilnosti vodnih raztopin metionina in glicil-metionina z uporabo tekočinske kromatografije = Stability testing of methionine and glycil-methionine in aqueous solution by liquid chromatography : diplomska naloga
Kušar, Julija
Metionin je esencialna aminokislina, katere tioetrni del je obèutljiv na oksidacijo. Ker je metionin pogosto sestavni del farmakolosko pomembnih peptidov in proteinov in oksidacija ena izmed glavnih ...razgradnih poti, igra oksidacija metionina pomembno vlogo pri nestabilnosti proteinskih uèinkovin. Nas namen je bil prouèiti lastnosti prostega in v dipeptid vezanega metionina in na podlagi dobljenih rezultatov napovedati obnasanje terapevtskih proteinov. Spremljanje stabilnosti izbranih modelnih spojin ima velik pomen, za kar pa potrebujemo selektivno, natanèno in toèno metodo. V nasem primeru smo uporabili HPLC z UV detekcijo. Na osnovi pripravljenih stresnih vzorcev smo razvili ustrezno analizno metodo in kasneje z vrednotenjem potrdili njeno primernost za doloèanje metionina in glicil-metionina v vodni raztopini. Hkrati smo ugotovili tudi moznost elektrokemiène detekcije obeh modelnih spojin. V okviru stabilnostne studije smo ugotovili, da je samostojen, kot tudi v dipeptid vkljuèen metionin obèutljiv na temperaturo, prisotnost kisika in svetlobo, oksidacija pa je odvisna predvsem od koncentracije, narave pufra in pH vrednosti ter prisotnosti kovinskih ionov, ki reakcijo oksidacije katalizirajo.
Type of material - undergraduate thesis
Publication and manufacture - Ljubljana : [Kušar, J.], 2004
Knjižnica dp 542 Kušar Julija Spremljanje IN: 0007640
available - reading room
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