Proučevanje fitoestrogenov in derivaov cimetne kisline kot inhibitorjev rekombinantnih človeških hidroksisteroid-dehidrogenaz AKR1C1 in AKR1C3 = Phytoestrogens and cinnamic acid derivatives as inhibitors of recombinant human hydroxysteroid dehydrogenases AKR1C1 in AKR1C3 : diplomsko delo
Bradač, Mateja, farmacevtka
Fitoestrogeni so nesteroidne spojine rastlinskega izvora in sestavni del nase prehrane. So naravni endokrini modulatorji, ki na veè naèinov vplivajo na kompleksni èlovekov endokrini sistem. Delujejo ...kot agonisti ali antagonisti estrogenskih receptorjev (ERŽ in ERŽ), uravnavajo pa tudi delovanje kljuènih encimov v biosintezi estrogenov kot je 17Ž-hidroksisteroid-dehidrogenaza (17Ž-HSD). Kljuèni gradnik fitoestrogenov je cimetna kislina. Estre cimetne kisline kot inhibitorje glivne 17Ž-HSD in fitoestrogene kot inhibitorje èloveske 17Ž-HSD tipa 5 so ze preuèevali, niso pa se testirali fitoestrogenov in derivatov cimetne kisline kot potencialnih inhibitorjev èloveske 20Ž-HSD. AKR1C1 (20Ž-HSD) in AKR1C3 (17Ž-HSD tip 5) sta encima za uravnavanje koncentracije androgenov, estrogenov in progestagenov. Rekombinantna AKR1C1 inAKR1C3 smo izrazili v E.coli v obliki fuzijskih proteinov z glutation-S-transferazo, oèistili z afinitetno kromatografijo na glutation sefarozi in izolirali s cepitvijo s trombinom. Kot potencialne inhibitorje obeh encimov smo testirali 18 fitoestrogenov in 20 derivatov cimetne kisline.AKR1C1 in AKR1C3 katalizirata NADPH odvisno redukcijo progesterona v 20Ž-hidroksiprogesteron in androstendiona v testosteron. Obe reakciji smo spremljali brez in v prisotnosti potencialnih inhibitorjev. Pri encimu AKR1C1 smo s pomoèjo spektrofotometra merili razliko v absorbanci NADPH, pri AKR1C3 pa smo s TLC metodo merili odstotek pretvorbe substrata. Izraèunali smo zaèetne hitrosti in doloèili IC50. Med sinteznimi derivati samo eden inhibira AKR1C3, med fitoestrogeni pa smo nasli veliko inhibitorjev obeh encimov. Za najboljsega inhibitorja AKR1C1 se je izkazal apigenin, AKR1C3 pa najbolje inhibira genistein.
Type of material - undergraduate thesis
Publication and manufacture - Ljubljana : [Bradač, M.], 2005
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
