Modulation of the expression of heat shock proteins and cytkins in Caco-2 cells after exposure to L. gasseri LF221, L. sakei NCDO 2174 and some microbial products
Fajdiga, Sana ...
To investigate whether lactobacilli (Lactobacillus gasseri LF221 and Lactobacillus sakei NCDO 2174), Salmonella enteritidis 857 and some microbial products (lactate, butyrate) were able to modify the ...heat schock response and IL-8 production in 5 days old enterocyte-like Caco-2 cells, the levels of Hsp70 in Caco-2 cells exposed to lactobacilli, their microbial products and S.enteritidis 857 were analysed by Wester blotting and immunostaining. The IL-8 levels were determined by sandwich ELISA. The expression of Hsp70 was similar between the bacterial strains. S. enteritidis 857 induced the highest IL-8 levels. Whereas butyrate had the potential to induce the expression of Hsp70, lactate only slightly modulated this level. Both butyrate and lactate had litter or no effect at all on the IL-8 levels.
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