Encimsko modificirane beljakovine sirotke : magistrsko delo
Gramc-Livk, Andreja, 1965-
V delu je opisana priprava topnih hidrolizatov beljakovin sirotke s proteazama mikrobiološkega izvora različne specifičnosti (alkalaza in nevtraza) do določene stopnje hidrolize in njihova ...karakterizacija. Za karakterizacijo hidrolizatov beljakovin sirotke sem uporabila kombinacijo elektroforeznih in kromatografskih metod. Z gelsko permeacijsko tekočinsko kromatografijo visoke ločljivosti (GP-HPLC) in elektroforezo na poliakrilamidnem gelu z dodatkom SDS (SDS-PAGE) sem določila porazdelitev molskih mas peptidov v hidrolizatih, določitev prostih aminoskupin s trinitrobenzensulfonsko kislino sem uporabila za izračun povprečne dolžine peptidnih verig v hidrolizatih, nizkomolekulsko peptidno frakcijo, dobljeno s preparativno kolonsko kromatografijo, sem s tekočinsko kromatografijo na reverzni fazi (RP-HPLC) ločila na devet glavnih peptidnih frakcij.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [A. Gramc], 1997
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