Vpliv litija na celični liniji FRTL-5 in CHO ter na humane ščitnične folikle "in vitro" : doktorska disertacija
Gaberšček, Simona
Lithium is widely used in treatment and prophylaxis of recidivant bipolar psychiatric disorders. Lithium accumulates in thyroid gland and can cause goiter or thyroid disfunction. The mechanisms of ...various effects of lithium ion on thyroid cells have not been completely clarified. The aims of our work wereČ 1) to establish if lithium, in presence and absence of thyrotropin (TSH), stimulates the synthesis of cAMP (cyclic adenosine 3), 5) - monophosphate)č as model system we used a strain of rat follicular cells FRTL-5 (Fischer Rat Thyroid cells in Low serum) and a line of Chinese hamster ovary fibroblasts (CHO), 2) to confirm/refute the literature data on stimulation of selected lithium concentrations on growth of thyroid cells, using a 3H-thymidine incoiporation assay into DNA of FRTL-5 cells, and 3) to test if lithium can have cytotoxic effects on FRTL-5 and CHO cells, and on isolated human thyroid follicles, using cytotoxicity assay with 51Cr release. Lithium at 0.35mM, 0.7mM,1mM,1.4mM,1.7mM and 2mM concentractions with and without TSH increased significantly the cAMP synthesis in FRTL-5 and in CHO cells, when compared with controls without lithium. These results are different from published data, which either have not been able to confirm the influence of lithium on cAMP synthesis or have even reported inhibition of cAMP synthesis. In all published investigations, however, lithium with TSH wastested only. According to our results, we can assume that some effects of lithium are partly mediated true the G proteins and cAMP synthesis. In FRTL-5 cells, lithium at 0.7mM,1mM,1.4mM,1.7mM and 2mM concentrations without TSH andat 1mM and 1.7mM concentrations with TSH significantly stimulated 3H-thymidine incorporation into cell DNA. The results confirm in vivo studies of thyroid gland enlargement during lithium therapy. (Abstract truncated at 2000 characters.)
Type of material - dissertation
; adult, serious
Publication and manufacture - Ljubljana : [S. Gaberšček], 1999
doktorske disertacije GABERŠČEK Simona Vpliv IN: 099901013
available - outside loan, loan period: 1 months
MF, Central Medical Library, Lj.
doktorske disertacije GABERŠČEK Simona Vpliv IN: 099901014
available - outside loan, loan period: 1 months
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