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Ugotavljanje genomske mikrosatelitske nestabilnosti pri bolnikih s karcinomom prostate = Evaluation of genomic microsatellite instabiity in patients with prostate cancer : diplomska nalogaŠaula, JelenaRak je bolezensko stanje, ki je posledica motenj v stevilnih protoonkogenih, tumor supresorskih genih in genih sistema za popravljanje neujemanja baznih parov v DNA (sistem MMRč angl. mismatch ... repair)č v nalogi smo se osredotoèili na ugotavljanje vloge genov sistema MMR pri razvoju karcinoma prostate. Eden izmed posledic izgube funkcije popravljalnega mehanizma je pojav mikrosatelitske nestabilnosti (angl. microsatellite instability - MSI), ki jo opredelimo kot pojav novih alelov v tumorskem tkivu. Te spremembe so rezultat insercije ali delecije tandemskih ponovitev DNA in jih v tumorju zaznamo zaradi klonalnega prerasèanja spremenjenih celic. Nestabilnost v mikrosatelitskih zaporedjih je uporabna kot bioloski oznaèevalec za ugotavljanje vrste karcinoma, iz èesar lahko sklepamo na prognozo bolezni. Pomembna je tudi pri izbiri ustrezne terapije in omogoèa individualizirano terapijo.Odloèili smo se za analizo MSI v mononukleotidnih oznaèevalcih NR-21,NR-22, NR-24, BAT-25 in BAT-26, katerih dolzine zaporedij se znotraj populacije spreminjajo za 2 bpč zaporedja so kvazimonomorfna. Analizirali smo vzorce 110 bolnikov s karcinomom prostate. Stopnje malignosti karcinomov, zaradi pomanjkljivih podatkov, nismo mogli doloèiti.Oznaèevalce smo pomnozili v reakciji multiple verizne reakcije s polimerazo. Sintetizirane fragmente smoanalizirali s kapilarno elektroforezo in za vsakega od oznaèevalcev ugotovili dva prevladujoèa alela. Pri oznaèevalcu NR-21 sta to alela dolzin 102 bp in 103 bp, pri NR-22 140 bp in 139 bp, za NR-24 sta znaèilna 128 bp in 129 bp dolga alela. Pri BAT-25 smo ugotovili enak odstotek pojavljanja za alela dolzin 120 bp in 121 bp. Dolzini 115 bp in 116 bp sta bili znaèilni za BAT-26. Na podlagi izmerjenih dolzin alelov za posamezen oznaèevalec in njihove pojavnosti znotraj populacije smo doloèili kvazimonomorfno obmoèje za slovensko populacijo. Z doloèitvijo le-tega smo omogoèili uporabo metode za analizo in opredelitev stopnje mikrosatelitske nestabilnosti tudi za druge vrste malignih obolenj. Ugotovili smo, da so mononukleotidna zaporedja zelo stabilna, saj je bil nestabilen le oznaèevalec NR-24 v enem izmed tumorskih vzorcev, ki smo ga zato opredelili kot nizko nestabilen. Ostale vzorce karcinoma prostate smo opredelili kot mikrosatelitsko stabilne. Na podlagi rezultatov smo sklepali, da analizirani oznaèevalci niso informativni za ocenovloge pri razvoju karcinoma.Type of material - undergraduate thesisPublication and manufacture - Ljubljana : [J. Šaula], 2007Language - slovenianCOBISS.SI-ID - 2055537
Author
Šaula, Jelena
Other authors
Mlinarič-Raščan, Irena |
Toplak, Nataša, 1974-
Topics
prostata |
rak prostate |
mikrosateliti |
mikrosatelitska nestabilnost |
metode |
zdravkjenje karcinoma prostate
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Library | Call number – location, accession no. ... | Copy status |
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Faculty of Pharmacy, Lj. | Knjižnica dp 616 Šaula Jelena Ugotavljanje IN: 0009327 |
available - reading room |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Šaula, Jelena | ![]() |
Mlinarič-Raščan, Irena | 12443 |
Toplak, Nataša, 1974- | 22582 |
Source: Personal bibliographies
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