Vnos gena za človeški prokatepsin S v krompir, Solanum tuberosum L., sorte Desireé : magistrsko delo = Construction of transgenic potato plants, Solanum tuberosum L., cv. Desireé, carrying a human procathepsin S gene : master of science thesis
Stanič, Darja, biologinja
The idea of using plants to produce different compounds is no longer novelty. Proteins and peptides of animal and human origin, when expressed in plants are usually correctly folded and glycosylated, ...unlike in bacterial expression systems, where this frequently presents a problem. Cathepsin S is highly active lysosomal cysteine proteinase. The involvement of cathepsin S in the pathogenesis of degenerative diseases in humans renders it a possible target for therapy. Katepsin S also plays an immportant role in normal physiological processes. For studying its function and structure larger amounts of enzyme are needed. In order to overcome the problem of the limited amounts of chatepsin S available from tissues, its cloning and expression were undertaken. We used a plant expression system as an alternative to a bacterial expression system, where cathepsin S is deposited in an insoluble and inactive form as inclusion bodies. We transformed potato plants cv. Desireé with the procathepsin S gene under regulation of the CaMV promoter. The procathepsin S gene could be detected in all the tissues of potato plants in vitro and in vivo. We determined the number of procathepsin genes in different clones of transformed potato plants. We have also detected procathepsin S mRNA in those clones, but were unable to detect translational product, procatepsin S or its cleaved form, cathepsin S.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [D. Stanič], 1998
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