Vrstna sestava populacije kvasovk v moštu malvazije : diplomska naloga = Yeast population in Malvasia must : graduation thesis
Župec, Andreja, 1975-
Kvasovke imajo v proizvodnji vina pomembno vlogo.V našem eksperimentu smo želeli določiti vrstno sestavo populacije kvasovk v moštu malvazije takoj po fazah stiskanja in razsluzenja grozdja. Iz mošta ...smo izolirali 329 izolatov kvasovk. 238 izolatov smo analizirali z metodo PCR - ribotipizacije in na osnovi dobljenih restrikcijskih profilov analiyirane izolate razvrstili v osemskupin. Iz vsake skupine smo izbrali 1-5 predstavnikov za identifikacijo s pomočjo fizioloških in biokemijskih testov. Na 18 izolatih smo nato izvedli po pribliÍno 50 asimilacijskih testov, tri fermentacijske teste, tri teste na rast pri povišani temperaturi, dva testa na rast pri povišanem osmotskem pritisku, ugotavljali produkcijo ocetne kisline, odpornost proti cikloheksimidu, ureayno aktivnost, testirali na barvno reakcijo z reagentom DBB in preverjali sposobnost tvorbe psevdomicelija. Pregledovali smo tudi morfološke lastnosti celic in kolonij in ugotavljali prisotnost spor. Okoli 60% analiziranih izolatov smo identificirali kot vrsto Candida stellata, 20% kot vrsto Hanseniaspora uvarum in 12% kot vrsto Metschnikowia pulcherrima. Ostalih 8% populacije pripada petim vrstam kvasovk, ki jih nismo uspeli identificirati. Z metodo PCR - ribotipizacije smo potrdili pravilnost identifikacije vrst H. uvarum in M. pulcherrima.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [A. Župec] : [BF, Enota meddoddelčnega študija mikrobiologije], 1998
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