Identifikacija pigmentiranih kvasovk vinorodne dežele Primorska : diplomska naloga = Identification of pigmented yeasts from Primorska wine region : graduation thesis
Brezovec, Sebastjan
V Zbirki industrijskih mikroorganizmov Ljubljana (ZIM) se nahaja več izolatov pigmentiranih kvasovk. Izolati izhajajo iz vinorodne dežele Primorska in so bili pridobljeni jeseni leta 1994 in jeseni ...leta 1995. Izolati so posredno ali neposredno povezani z vinskim področjem saj izhajajo iz zemlje v vinogradih, površine grozdnih jagod, mošta in vinarske opreme. Namen diplomskega dela je bil identificirati rodove in vrste rdeče pigmentiranih kvasovk iz omenjenih habitatov. Za identifikacijo so nam služili fiziološki testi: (i) rast na gojiščih, kjer naslednje spojine predstavljajo edini vir C oz. N: L-arabinoza, L-arabinitol, citrat, D-glukuronat, laktoza, L-lizin, maltoza, melezitoza, nitrat, rafinoza, saharoza, salicin in škrob, (ii) rast na gojiščih brez vitaminov: p-aminobenzojske kisline in tiamina in (iii) sposobnost rasti pri 25 °C in pri 30 °C. Tipske in nekatere že identificirane seve sem uporabljal kot referenčne mikroorganizme. S programom za identifikacijo kvasovk Barnetta s sod. (1996) sem identificiral 17 izolatov, ki pripadajo naslednjim vrstam: Bullera variabilis (1), Rhodotorula aurantiaca (1), Rhodotorula minuta (3), Rhodotorula mucilaginosa (9), Sporidiobolus johnsonii (1), Sporidiobolus pararoseus (2), Sporobolomyces roseus (1). Upoštevajoč morfologijo kolonij in tvorbo balistospor sklepam, da neidentificirani izolat ZIM 1017 pripada balistosporogenim kvasovkam. Lahko bi ga identificiral kot kvasovki Bullera variabilis ali Sporidiobolus pararoseus.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [BF, Oddelek za živilstvo] : [S. Brezovec], 1999
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