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Strukturne in evolucijske analize Bov-B LINE elementov pri vretenčarjih : doktorska disertacijaŽupunski, VeraBov-B LINEs are non-LTR retrotransposons that transpose via an RNA intermediate in the genome and between genoms. Non-LTR retrotransposons are divided into 16 clades and Bov-B LINEs are members of ... the RTE clade. The distribution and evolutionary dynamics of the Bov-B LINE element and other non-LTR retrotransposons from the RTE clade have been examined. We have determined the sequence of the full-length Bov-B LINE from Vipera ammodytes, the first full-lenght retrotransposon from the RTE clade in Squamata to be reported. The element has a structural organisation typical of the RTE clade of non-LTR retrotransposons. The distribution of Bov-B LINEs in vertebrates and invertebrates was examined by amplification of a major part of the open reading frame ORF2, which encodes two crucial enzymes for retrotransposition. Bov-B LINEs are present in squamates, wallaby (Macropus) and ruminants. Intraspecies and interspecies variability was very low. An unusually high level of sequence conservation (83 %) also exists between the evolutionarily older snakes and Ruminantia. The distribution of the RTE retrotransposons was examined by searching a number of databases. The RTE clade has been found to be much more widely distributed than previously thought, and novel representatives have been discovered in plants, brown algae, annelids, crustaceans, mollusks, echinoderms and teleost fishes including the full-length RTE elements, Bov-BžRTE from Bombyx mori and Strongylocentrotus, plant RTE and Oryzias RTE. They all have the conserved structural organisationof the RTE clade. Analysis of their sequences has shown that Bov-BLINEs originated in invertebrates, from which the element was transmittedhorizontally to the reptiles. In squamates, amplification and vertical transmission has been observed. In the genome of the long-nosed vipersome active copies of the Bov-B LINE may still be present. (Abstract truncated at 2000 characters).Type of material - dissertation ; adult, seriousPublication and manufacture - Ljubljana : [V. Župunski], 2001Language - slovenianCOBISS.SI-ID - 2284052
Author
Župunski, Vera
Other authors
Gubenšek, Franc, 1937-2010 |
Križaj, Igor
Topics
Retroelements |
Chemistry |
Retroelements |
Genetics |
Vertebrates |
Genetics |
Evolution, molecular |
Retroelementi |
Kemija |
Retroelementi |
Genetika |
Vretenčarji |
Genetika |
Evolucija, molekularna |
Retrotranspozoni |
Vretenčarji |
Disertacije |
molekularna genetika |
genom |
transpozicijski elementi |
nukleotidno zaporedje |
molekularna evolucija
Library | Call number – location, accession no. ... | Copy status |
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National and University Library, Ljubljana | GS II 525633 glavno skladišče | available - reading room |
MF, Central Medical Library, Lj. | doktorske disertacije ŽUPUNSKI Vera Strukturne IN: 020010580 |
available - outside loan, loan period: 1 months |
MF, Central Medical Library, Lj. | doktorske disertacije ŽUPUNSKI Vera Strukturne IN: 020010579 |
available - outside loan, loan period: 1 months |
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Database name | Field | Year |
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Župunski, Vera | 15640 |
Gubenšek, Franc, 1937-2010 | 01083 |
Križaj, Igor | 00412 |
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