Lokalizacija nekaterih proteinov kot fuzije s proteinom GFP v kvasovki Saccharomyces cerevisiae : diplomska naloga = Localisation of some proteins as GFP protein fusions in yeasts Saccharomyces cerevisiae : graduation thesis
Manček, Mateja
Zeleno fluorescirajoři protein (GFP) je protein, ki ga uporabljamo kot porořevalsko molekulo za zasledovanje genske ekspresije in proteinsko lokalizacijo in vivo ter in situ skozi vse faze celiřnega ...cikla. Sam protein GFP nima signala za lokacijo, zato se nahaja v citoplazmi. če proteinu v řitalni okvir vstavimo signalno zaporedje, ga lahko lokaliziramo tudi v organelih. S pomořjo GFP-ja smo poskuǴali lokalizirati nekatere proteine, ki vsistemu dvo-hibridne interakcije interagirajo s proteinom Cdc6: Ecm11 in Ty1A. Protein Ecm11 se nahaja v jedru v vseh fazah celiřnega cikla. Obstaja verjetnost, da proteina dejansko interagirata v Íivi celici. Protein Ty1A se nahaja v citoplazmi v vseh fazah celiřnega cikla. Fuzijski protein Ty1A-GFP, kjer je GFP na C-terminalnem koncu, tvori VLP-je in inkluzijska telesca, ře gaizraÍamo iz mořnega promotorja GAL1. Inkluzijska telesca verjetno nastanejo zaradi prekomernega izraÍanja, kajti ob dodatku glukoze se raztopijo in sestavijo se VLP-ji. Fuzijski protein GFP-Ty1A, kjer je GFP na N-terminalnem koncu, tvori inkluzijska telesca, ki se ob dodatku glukoze raztopijo, vendar VLP-ji ne nastajo.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [M. Manček] ; [BF, Enota medoddelčnega študija mikrobiologije], 2000
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