Izolacija in karakterizacija 6-fosfofruktokinaze pri glivi Aspergillus niger : diplomska naloga = Isolation and characterization of 6-phosphofructokinase from Aspergillus niger : graduation thesis
Smerkolj, Sava
6-fosfofrukto-1-kinaza je eden kljuřnih regulatornih encimov glikolize. Ćelelismo ugotoviti, ali se pri sevu A60 glive Aspergillus niger pojavlja v veř oblikah in te oblike okarakterizirati. Glivo ...smo gojili v dveh razliřnih gojiǴřih in v enem primeru izvrǴili hipoosmotski Ǵok. Z gelsko filtracijo smo lořili dve po velikosti razliřni obliki PFK1 in ugotovili, da se lořita tudi po kinetiki v odvisnosti od ATP. S pomořjo frakcioniranega obarjanja smo prav tako lořili dve obliki in ugotovili, da se razlikujeta po stanju fosforiliranosti in kinetiki. Z afinitetno kromatografijo smo ořistili obe obliki encima in nato z lořitvijo s poliakrilamidno gelsko elektroforezo dolořili njuni molski masi, ki sta okoli 48 in 84 kDa. Zakljuřili smo, da se verjetno veřja oblika encima fosforilira preko cAMP-odvisne proteinske kinaze,na ta nařin postane nestabilna, razpade do manjǴe oblike in tako tudi spremeni svojo kinetiko.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [S. Smerkolj] ; [BF, Enota medoddelčnega študija mikrobiologije], 2000
National Institute of Biology and BF, Department of Biology, Ljubljana
BF oddelek za biologijo dn 579 SMERKOLJ S. Izolacija IN: 0032032
available - reading room
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