Vpliv dejavnikov okolja na izražanje gena traJ in na frekvence konjugacije plazmida pRK100 : diplomska naloga = Effect of environmental factors on expression of traJ gene and on frequency of conjugation of plasmid pRK100 : graduation thesis
Brinc, Matjaž
TraJ je poglavitni pozitivni regulator izražanja operona tra plazmida F Escherichia coli in aktivira prepisovanje iz promotorja traY. Z gensko fuzijo PtraJ-lacZ je bil določen vpliv nekaterih ...dejavnikov okolja in gostitelja na aktivnost promotorja traJ. Ugotovljen je bil tudi vpliv teh dejavnikov na frekvenco konjugacije F-podobnega plazmida pRK100. Aktivnost promotorja traJ in frekvenca konjugacije je obratno sorazmerna koncentraciji glukoze v gojišču. Mutacije v genih gostitelja sfrA in cpxA zmanjšajo aktivnost promotorja traJ in frekvenco konjugacije, mutacija v cpxR pa nanju ne vpliva. Koncentracija aminokislin in fosfata, dodajanje levcina in zamenjava glukoze z drugim virom ogljika prav tako nimajo vpliva na izražanje gena traJ.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [M. Brinc] : [BF, Enota medoddelčnega študija mikrobiologije], 2000
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