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Chemiluminescence of non-differentiated THP-1 promocytes : developing an assay for screening anti-inflammatory milk proteins and peptidesTompa, Gorazd ...Human leukemic THP-1 promonocytes are widely used as a model for peripheral blood monocytes. However, superoxide production during respiratory burst (RB) of non-differentiated THP-1 (nd-THP-1) cells ... is very low. Here we present a rapid and low-cost method for measuring the chemiluminescence (CL) of opsonized zymosan (OZ) induced RB which allows detection of Escherichia coli lipopolysaccharide (LPS) induced priming of nd-THP-1 cells on the basis of CL reaction kinetics. Maximum CL intensity obtained was 2.20+- 0.25 and 1.30+- 0.11 relative light units, while CL peak time was achieved at 18.1+- 2.6 and 28.7+- 1.3 min in primed and non-primed cells, respectively. The priming of nd-THP-1 cells with LPS evoked tzpical TNF-[alfa] and IL-6 production. We tested the effects of bovine lactoferrin and protein fractions from Lactobacillus helveticus BGRA43 fermented milk for potential anti-inflammatory effects on LPS primed nd-THP-1 cells. Four fractions were found to inhibit the OZ-induced CL in a dose-dependent manner (IC50 3-30 g/mL), while lactoferrin inhibited CL to a lesser extent (IC50 270? g/mL). These results suggest that measuring CL response of nd-THP-1 cells can serve as a method for screening anti-inflammatory compounds which could be used in reducing the risk of phagocyte-mediated inflammatory diseases.Source: Luminescence. - ISSN 1522-7235 (Vol. 26, no. 4, 2011, str. 251-258)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 2757768
Author
Tompa, Gorazd |
Laine, Aire |
Pihlanto, Anne |
Korhonen, Hannu, 1945- |
Rogelj, Irena, 1955- |
Marnila, Pertti
Topics
mleko |
beljakovine |
peptidi |
analizna kemija |
kemiluminiscenca
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Tompa, Gorazd | 27549 |
Laine, Aire | |
Pihlanto, Anne | |
Korhonen, Hannu, 1945- | |
Rogelj, Irena, 1955- | 08857 |
Marnila, Pertti |
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