Identifikacija težje določljivih vrst netuberkuloznih mikobakterij izoliranih iz humanih kužnin : diplomsko delo, univerzitetni študij = Identification of rarely isolated non-tuberculous mycobacterial species from clinical specimens : graduation thesis, university studies
Kržišnik, Jerneja, mikrobiologija, 1979-
The rapid and accurate diagnosis of mycobacteria is important for correct treatment of patients. For many years, the identification has relied on investigation of culture properties and biochemical ...characteristics. Conventional biochemical methods are timeconsuming. It was therefore decided to investigate an identitication system based on DNA technology. There are many molecular mcthods for identification of mycobacteria, but none of thcm are suitable for identification of rarely isolated species of non-tuberculous mycobacteria. For rapid identification of this kind of species a newly developed assay GenoType Mycobacterium AS (Hain Lifescience, Nehren, Germany) was evaluated. The assay is able to identify 16 more rarely isolated mycobacterial species. The test is based on polymerse chain reaction followed by hybridisation to specific oligonucleotide probes immobilized on membrane strips. The conventional biochemical identification methods are currently onlymethod used in Slovenia for identification of rarely isolated non-tuberculous mycobacterial species. fhe aim of our study was comparative evaluation of two methods for identification of mycobacterial speciesČ conventional biochemical methods versus molccular assay GenoType MyeobacteriumAS. In the study we included 47 samples, all from Slovenia. Firstwe tried to identify samples with conventional biochemical methods. Then we repeated identification of samples with molecular assay. The results were compared. The molecular assay identified morc samplcs to the species level then conventional biochemical methods, 6 (13%) versus 1 (2%). The advantages of the assay is that provides identification of a large variety of rarely isolated nontubercuolus mycobacterial species in a single test, it can identify multiple mycobacterial species within the samc sample, is rapid and the results are easy to interpret.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [J. Kržišnik] : [BF, Enota medoddelčnega študija mikrobiologije], 2005
It was not necessary to add the material to the shelf.
Bibliographic material was successfully added on shelf.
Adding bibliographical material on shelf was not successful.
Material is already on the shelf.
Permalink
URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year
Impact factor
Edition
Category
Classification
JCR
SNIP
JCR
SNIP
JCR
SNIP
JCR
SNIP
Select the library membership card:
DRS,
in which the journal is indexed
Database name
Field
Year
Links to authors' personal bibliographies
Links to information on researchers in the SICRIS system
Subject headings in COBISS General List of Subject Headings
Select pickup location
The material from the parent unit is free. If the material is delivered to the pickup location from another unit, the library may charge you for this service.
