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  • Methotrexate reduces HbA1c concentration but does not produce chronic accumulation of ZMP in patients with rheumatoid or psoriatic arthritis
    Perdan-Pirkmajer, Katja ...
    Objectives: The mechanism by which methotrexate (MTX) improves glucose homeostasis in patients with rheumatoid (RA) and psoriatic arthritis (PsA) remains undetermined. Animal studies indicate a role ... for intracellular accumulation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5-monophosphate (ZMP) but this has not been directly demonstrated in humans. We explored whether accumulation of ZMP is associated with improvements in glucose homeostasis during MTX therapy. Method: MTX-naïve, non-diabetic RA (n = 16) and PsA (n = 10) patients received uninterrupted MTX treatment for 6 months. To evaluate whether ZMP accumulated during MTX therapy, we measured the concentration of ZMP in erythrocytes and the concentration of its dephosphorylated derivative 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR) in urine using liquid chromatography mass spectrometry (LC-MS/MS). To assess glucose homeostasis, we determined the concentration of glycated haemoglobin (HbA1c) and homeostasis model assessment of insulin resistance [HOMA-IR: fasting glucose (mmol/L) x fasting insulin (microU/mL)/22.5]. Results: Erythrocyte ZMP and urinary AICAR concentrations did not increase during 6 months of MTX therapy. HbA1c concentration was reduced from 5.80 +- 0.29% at baseline to 5.51 +- 0.32% at 6 months (p < 0.001), while HOMA-IR remained unaltered. Reduction in HbA1c concentration was not associated with increased ZMP or AICAR concentrations. Conclusions: MTX therapy probably does not produce a chronic increase in erythrocyte ZMP or urinary AICAR concentrations. Collectively, our data do not support the hypothesis that MTX improves glucose homeostasis through chronic accumulation of ZMP.
    Source: Scandinavian journal of rheumatology. - ISSN 0300-9742 (Vol. 45, iss. 5, Oct. 2016, str. 347-355)
    Type of material - article, component part ; adult, serious
    Publish date - 2016
    Language - english
    COBISS.SI-ID - 32413401

    Link(s):

    http://www.tandfonline.com/doi/full/10.3109/03009742.2015.1105290
    DOI

source: Scandinavian journal of rheumatology. - ISSN 0300-9742 (Vol. 45, iss. 5, Oct. 2016, str. 347-355)

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