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Fungi gene expression systems [Elektronski vir] : applications and advancementsKraševec, Nada ; Benčina, Mojca, 1968-Due to their capacity for producing large amounts of secreted proteins, filamentous fungi have a long history of industrial use for the production of endogenous enzymes. While the genetic engineering ... tools developed for filamentous fungi expanded the use of fungi for the production of heterologous proteins, the production of recombinant proteins is often limited. Low-cost, high-throughput DNA sequencing technologies, quantitative physiology, metabolic engineering, systems biology, and the emerging interest for clean, renewable carbon sources have accelerated the focus of fungal research towards studies that seek to investigate ways to improve recombinant protein production. In this chapter, we evaluate the advantages and disadvantages of bacteria, yeast, filamentous fungi, and higher eukaryotes as hosts for recombinant protein production on an industrial scale. The role of filamentous fungi as hosts for protein production in structural biology is questioned. We also discuss the emerging opportunities offered by the complementary fields of systems biology and synthetic biology, which might open new possibilities for successful heterologous protein production in fungi.Source: Gene expression systems in fungi [Elektronski vir] : advancements and applications (Str. 201-226)Type of material - e-articlePublish date - 2016Language - englishCOBISS.SI-ID - 38441477
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Kraševec, Nada | 00385 |
Benčina, Mojca, 1968- | 14360 |
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