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Analizna metoda za določanje vitamina B12 v vzorcih fermentirane kisle sirotke [Elektronski vir] = Analytical method for vitamin B12 determination in fermented acid wheyPlaninšek Parfant, Timeja ...Cianokobalamin (CNCbl) je najstabilnejša in najpogosteje uporabljena oblika vitamina B12. Zaradi kompleksne strukture se ga pridobiva s fermentacijo z bakterijami, kjer se lahko kot gojišče uporablja ... tudi kisla sirotka. Za namen ugotavljanja uspešnosti fermentacije smo razvili analizno metodo za določanje vitamina B12 v vzorcih, pridobljenih s fermentacijo kisle sirotke. Ugotovili smo, da je za sprostitev vitamina B12 iz bakterijskih celic ključno vsaj 10 minutno avtoklaviranje pri 121 °C. Za pretvorbo kobalaminov naravnega izvora v CNCbl pa je optimalen dodatek 0,1 % KCN v 0,2 M acetatnem pufru s pH vrednostjo 4,5 v razmerju z vzorcem 1:1. Vzorce smo analizirali s predhodno razvito in validirano instrumentalno LC-MS/MS metodo.Type of material - conference contribution ; adult, seriousPublish date - 2022Language - slovenian, englishCOBISS.SI-ID - 99173379
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Planinšek Parfant, Timeja | 52446 |
Osel, Nika, farmacevtka | 53673 |
Kristl, Albin | 11122 |
Paveljšek, Diana | 36376 |
Bogovič Matijašić, Bojana | 11150 |
Roškar, Robert, 1975- | 23549 |
Source: Personal bibliographies
and: SICRIS
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