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Pridobivanje nizina in vitamina B12 z biokonverzijo kisle sirotke [Elektronski vir] = Production of nisin and vitamin B12 by acid whey bioconversionPaveljšek, Diana ...Optimizirali smo produkcijo nizina in vitamina B12 v bioreaktorjih različnega volumna. Preizkusili smo več metod koncentriranja, čiščenja ter analitike ugotavljanja nizina. Produkcija nizina je bila ... boljša, kadar smo med fermentacijo uravnavali pH nad 6, se je pa v tem primeru večji del nizina vezal na bakterije (pelet) (skoraj 50 %). Tako pridobljeni nizin lahko z nižanjem pH pod 3 sprostimo s celic v gojišče. Za koncentriranje nizina na večjem nivoju je tako najbolj primeren način ultrafiltracija, v kombinaciji z uravnavanjem pH. Nizin smo poskušali izolirati tudi s penjenjem z detergentom in nadaljnjo izolacijo z mešanico metanola in acetona. S penjenjem ob dodatku detergenta lahko nizin dodatno skoncentriramo, sama izolacija z metanolom in acetonom pa je primerna za pridobivanje bolj čiste oblike nizina. Vitamin B12 smo proizvajali v dveh stopnjah in na koncu fermentacije poželi bakterijske celice s centrifugiranjem ter iz njih sprostili vitamin B12, ki smo ga merili z LC-MS/MS.Type of material - conference contribution ; adult, seriousPublish date - 2022Language - slovenian, englishCOBISS.SI-ID - 99177219
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DRS, in which the journal is indexed
Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Paveljšek, Diana | 36376 |
Oberčkal, Jernej | 33324 |
Planinšek Parfant, Timeja | 52446 |
Osel, Nika, farmacevtka | 53673 |
Roškar, Robert, 1975- | 23549 |
Bogovič Matijašić, Bojana | 11150 |
Source: Personal bibliographies
and: SICRIS
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