Carbon fraction (CF) of trees is essential for quantifying forest carbon (C) stocks. Considerable attention has been paid to CF variations at various levels with the exception of inter- and intra-growth rings. Herein, the inter- and intra-growth ring variation of CF in was investigated. Elemental analysis was performed to obtain CF values of the earlywood and latewood in each growth ring of the xylem. Patterns of CF variation at the growth ring level were evaluated using mixed-effect models. The results showed that latewood CF, 50.6 %, was significantly higher than earlywood CF, 49.9 % (  < 0.01). In particular, inter-growth ring variations of CF differed between heartwood and sapwood, as well as between juvenile wood and mature wood. CF values decreased nonlinearly with cambium age toward the heartwood or juvenile wood, with estimated least-squares means of 50.4 % and 51.8 %, respectively. While CF values were almost unaltered in sapwood, and slightly decreased in mature wood, with estimated least-squares means of 50.0 % and 50.2 %, respectively. It indicates that patterns of CF variation between juvenile wood and mature wood are important to estimate the C stock of . This research provides insights into C uptake dynamics to support forest management and wood utilization.