As a procedural best practice, it is recommended that PCR products are cleaned up following initial amplification and prior to Sanger Sequencing. The goal of PCR product cleanup is to eliminate leftover primers and nucleotides that interfere with the sequencing reaction and lead to poor results. A speedy and efficient way to do this is with enzymes that digest excess PCR primers and degrade unincorporated nucleotides. The most effective and well referenced product for this is ExoSAP-IT reagent from Affymetrix(R), which combines Exonuclease I primer digestion and Shrimp Alkaline Phosphatase nucleotide degradation into a single cleanup step. HT ExoSAP-IT Fast High-Throughput PCR Product Cleanup is the ideal solution in high volume labs with quick sample turnaround requirements. HT ExoSAP-IT Fast reagent has a 14 minute total protocol time, which is over 50% faster than standard ExoSAP-IT reagent. It is added directly to the PCR product for a 7 minute cleanup at 37degreesC, followed by a 7 minute inactivation at 80degreesC.