•Possible bio- applications of bistable glass-coated microwires are shown.•Biocompatibility was proven experimentally.•Application on temperature sensing in cranium implants is confirmed.•Application for osteomalacy monitoring is shown. In the given contribution, two examples of applications of glass-coated microwires for biomedical sensing are shown. Firstly, application of microwires for contactless sensing of the intracranial temperature below titanium implants confirms the possibility to sense the temperature with sensitivity down to 0.01 °C. Secondly, application of microwires for monitoring the stress applied on bones after injury or for monitoring the osteomalacy effect is shown. Our measurements confirm that high sensitivity of order of 10−10 s/Pa can be obtained.