The synthesis of silver nanoparticles has gained interest due to their diverse applications in various fields. It is very essential to use capping agents to avoid loss of nanosized characteristics due to agglomeration of AgNPs. In the present investigation, a simple and environmentally viable protocol to synthesize a wide range of concentrations of non-agglomerated and colloidal AgNPs using extracts of discarded wastes (gills and intestine) of Tilapia (Oreochromis mossambicus) in three different aqueous media without the requirement of any additional stabilizing and capping agent is described. The size of most of AgNPs ranged from 2 to 20 nm, followed by sizes of 20-40 nm and some with sizes of 40-50 nm. Zeta potential was in the range of −31.1 to −52.8 mV and −33.3 to −53.6 mV, and surface plasmon resonance ranged from 405 to 410 nm for gill and intestine extracts respectively. HR-TEM images indicated that Phosphate buffer saline is the best suitable for synthesizing non-agglomerated AgNPs capped with biomolecules. Valorization has been achieved by trapping Ag NPs into two different forms of natural zeolites namely stilbite and mordenite and their ammonia removal efficiency have been evaluated. In addition, the antimicrobial activity of synthesized AgNPs and their valorized products exhibited potent inhibitory activity against test bacteria such as Escherichia coli and Aeromonas hydrophila. This study not only establishes a simple and environmentally viable protocol for the synthesis of AgNPs and valorized products for environmental and disease management but also adds to the utilization of fish wastes in solid waste management.