Neat and arginine modified montmorillonite, Mt and Mt-Arg, were embedded into polysulfone (PSf) to prepare adsorptive mixed matrix membranes (AMMMs) for arsenate removal from contaminated water. Prepared membranes were characterized by FE-SEM, XRD, AFM, pure water flux and contact angle measurement. Equilibrium adsorption capacity and adsorption kinetic of AMMMs were determined using batch adsorption experiments. In addition, dynamic adsorption and regeneration studies were also investigated in dead-end filtration setup. Adsorption kinetic of arsenate was found to follow pseudo-second-order kinetic model and equilibrium data showed good correlation with the Langmuir model for AMMMs. The obtained results revealed that the arsenate adsorption was favorable for PSf/Mt-Arg. Regeneration capability of the Mt-Arg embedded PSf membranes was assessed by conducting ten cycles of adsorption-desorption experiments. Arg modified Mt provides interesting properties to adsorb As(V) from water in neutral pH and desorb in alkaline condition; pH=9, due to negative side group. The obtained results confirmed the applicability of the prepared PSf/Mt-Arg in As(V) removal for multiple cycles. Display omitted •Arginine modified Mt was incorporated into PSf to prepare adsorptive membranes.•Adsorptive membranes were used for As(V) removal from water.•PSf/Mt-Arg membrane showed higher adsorption capacity than PSf/Mt membrane.•Mt-Arg provides interesting properties to adsorb and desorb As(V).•PSf/Mt-Arg membrane was re-generable and easy for multiple applications.