•Movement centered examination strategies for foot and ankle pathology can enhance clinical reasoning within the interprofessional team.•Dorsiflexion range of motion can impact movement patterns throughout the lower extremity kinetic chain.•Professionals managing foot and ankle pathology may consider regional interdependence applications of dorsiflexion range of motion limitations and the implications of movement dysfunction within the squat pattern. An association between limited ankle closed kinetic chain dorsiflexion range of motion (CKCDFROM) and movement dysfunction in the lower quarter is often implied, limited research exists linking CKCDFROM and gross movement patterns, such as the squatting. The purpose of this study is to investigate the association between CKCDFROM and movement patterns in collegiate athletes, as measured by the functional movement screen (FMS). A quasi-experimental observational analytical cohort study with 147 athletes from five Division III collegiate men’s and women’s athletic teams were included in the study. CKCDFROM was assessed utilizing the lunge test. Movement patterns, specifically the deep squat (DS) and inline lunge (ILL) were assessed utilizing the FMS qualitative criteria. Descriptive statistical analysis examined the association between CKCDFROM limitations and a dysfunctional deep squat or ILL. Seventy-nine (53.7%) and 30 (20.4%) participants scored a “1” on their FMS deep squat test and ILL, respectively. Participants who scored a “1” on the deep squat and ILL were 3.75 times as likely (3.75 odds ratio; 95% CI 1.57–9.14; p = 0.002) and 1.53 times as likely (1.53 odds ratio; 95% CI 0.65–3.60; p = 0.392), respectively, to have at least one ankle CKCDFROM limitation. The deep squat (DS) was statistically significant, but the ILL did not rise to the level of significance. Physical therapists should consider regional interdependence implications of movement dysfunction stemming from impairments within the kinetic chain. A dysfunctional lower extremity movement pattern might be associated with a lack of CKCDFROM. Clinicians will likely benefit from assessing CKCDFROM in those exhibiting dysfunctional squatting and/or lunging.