E-resources
Peer reviewed
-
Zhu, Yaolei; Sang, Xue; Li, Xue; Zhang, Yanan; Hao, Hongshun; Bi, Jingran; Zhang, Gongliang; Hou, Hongman
Applied microbiology and biotechnology, 09/2020, Volume: 104, Issue: 17Journal Article
The serp gene codes for a protease that is considered to be an important factor associated with quorum sensing (QS)–based food spoilage caused by microorganisms. In this study, we evaluated the effect of temperature (4–37 °C) and QS inhibitors on the production of N -acyl-L-homoserine lactones (AHLs) and relative expression of the luxR/I , as well as serp in Hafnia alvei H4. Production of AHLs and expression of luxR/I were found to reach maximum levels at 10 °C, suggesting that the QS system of H. alvei H4 might have higher activity at low temperatures; similar result was also obtained for serp expression. Mutants of H. alvei H4 deficient in QS were used to identify the regulation of QS on serp expression. Significant reduction ( P < 0.05) in serp expression was found in the mutants ∆ luxR , ∆ luxI , and ∆ luxR/I , with ∆ luxI and ∆ luxR/I showing greater reduction than ∆ luxR . Minimum inhibition concentrations (MIC) of Benzyl isothiocyanate and 3-Methylthiopropyl isothiocyanate for H. alvei H4 were determined to be 7.813 and 15.625 mM, respectively. Furthermore, the expression of serp , as well as that of luxR and luxI , was significantly repressed ( P < 0.05) by the two QS inhibitors at 1/8 MIC and 1/16 MIC, indicating that these inhibitors might repress serp expression through affecting luxR and luxI expression in H. alvei H4. The findings of this study, therefore, suggested that food spoilage caused by H. alvei could be controlled through the application of QS inhibitors.
Author
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.