The study aimed to compare the physical demands required during the first, second, and third most demanding passages (MDP) of play considering the effect of playing position, type of passage, and passage duration. A longitudinal study for three mesocycles was conducted in a professional soccer team competing in LaLiga123 . Tracking systems collected total distance covered (DIS), high-speed running distance (HSRD), sprinting distance (SPD), total of high-intensity accelerations (ACC HIGH ), and total of high-intensity decelerations (DEC HIGH ). The results confirmed that a significant effect of the type of passage (first, second or third MDP of play) on DIS (F (1.24, 178.89) = 115.53; p = 0.01; ηp 2 = 0.45), HSRD (F (1.35, 195.36) = 422.82; p = 0.01; ηp 2 = 0.75), SPD (F (1.43, 206.59) = 299.99; p = 0.01; ηp 2 = 0.68), ACC HIGH (F (1.45, 209.38) = 268.59; p = 0.01; ηp 2 = 0.65), and DEC HIGH (F (1.45, 209.38) = 324.88; p = 0.01; ηp 2 = 0.69) was found. In addition, a significant interaction between playing position, type and duration of the passage was observed in DIS (F (12.60, 453.47) = 1.98; p = 0.02; ηp 2 = 0.05) and ACC HIGH (F (13.99, 503.78) = 1.92; p = 0.03; ηp 2 = 0.06). In conclusion, significant differences in physical demands between the first, second, and third MDP of play were observed. However, there were some cases (DIS and ACC HIGH ) in which no significant differences were found between these passages. Therefore, coaches should consider not only the magnitude of these peak intensity periods (e.g., distance covered per minute) but also the number of passages that players may experience during match play.