The Mental Health Continuum-Short Form (MHC-SF) is a multidimensional instrument designed to capture emotional, psychological, and social well-being. It is one of the self-report measures of international use in clinical practice and research, although so far it has no validation in Spanish-Speaking adolescents. Therefore, the objective of this study was to analyze the reliability and validity evidence (structure, convergent and criterion), and the temporal and gender invariance of the MHC-SF in Spanish adolescent population. Two assessment moments with a 6-month time interval were used, with an initial sample of 5,479 adolescents and a later sample of 2,129. The CFA showed optimal fit for the bi-factor model, and an adequate fit for correlated three-factor model. The results of the gender and temporal invariance analysis showed optimal fit. Reliability coefficients were all higher than .77. The MHC-SF presented significant positive associations (p < .001) with indicators of well-being (r > .60) and negative associations with indicators of psychological distress (r > -.21). The MHC-SF shows evidence of reliability and validity in Spanish adolescents, being the bi-factor model invariant through time and across gender groups.