OBJECTIVES: To assess oncology nurses' awareness of biomarker testing and targeted therapy for ovarian cancer. SAMPLE & SETTING: 100 oncology nurses completed an online survey in June 2022. METHODS & VARIABLES: A cross-sectional survey was used to examine nurses' understandingof ovarian cancer testing and treatments, assess barriers, and identify opportunities for further education. RESULTS: Almostall respondents believed biomarker testing and targeted therapy were very/extremely important in diagnosing and supporting treatment of patients with ovarian cancer. Nurses were very/extremely familiar with cancerantigen 125 and germline testing, but fewer reported the same familiarity with somatic testing. Most nurses were familiar with targeted therapy for ovarian cancer, but only about half were very/extremely familiar with poly(ADP-ribose) polymerase (PARP) inhibitors. Less than half felt highly knowledgeable about PARP inhibitors. IMPLICATIONS FOR NURSING: It is important that oncology nurses understand biomarker testing and targeted therapy. There is an opportunity to provide resources to nurses to help them become more comfortable with PARP inhibitors in particular. KEYWORDS oncology nursing; ovarian neoplasms; biomarkers; PARP inhibitors