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Bowden, John A.; Heckert, Alan; Ulmer, Candice Z.; Jones, Christina M.; Koelmel, Jeremy P.; Abdullah, Laila; Ahonen, Linda; Alnouti, Yazen; Armando, Aaron M.; Asara, John M.; Bamba, Takeshi; Barr, John R.; Bergquist, Jonas; Borchers, Christoph H.; Brandsma, Joost; Breitkopf, Susanne B.; Cajka, Tomas; Cazenave-Gassiot, Amaury; Checa, Antonio; Cinel, Michelle A.; Colas, Romain A.; Cremers, Serge; Dennis, Edward A.; Evans, James E.; Fauland, Alexander; Fiehn, Oliver; Gardner, Michael S.; Garrett, Timothy J.; Gotlinger, Katherine H.; Han, Jun; Huang, Yingying; Neo, Aveline Huipeng; Hyötyläinen, Tuulia; Izumi, Yoshihiro; Jiang, Hongfeng; Jiang, Houli; Jiang, Jiang; Kachman, Maureen; Kiyonami, Reiko; Klavins, Kristaps; Klose, Christian; Köfeler, Harald C.; Kolmert, Johan; Koal, Therese; Koster, Grielof; Kuklenyik, Zsuzsanna; Kurland, Irwin J.; Leadley, Michael; Lin, Karen; Maddipati, Krishna Rao; McDougall, Danielle; Meikle, Peter J.; Mellett, Natalie A.; Monnin, Cian; Moseley, M. Arthur; Nandakumar, Renu; Oresic, Matej; Patterson, Rainey; Peake, David; Pierce, Jason S.; Post, Martin; Postle, Anthony D.; Pugh, Rebecca; Qiu, Yunping; Quehenberger, Oswald; Ramrup, Parsram; Rees, Jon; Rembiesa, Barbara; Reynaud, Denis; Roth, Mary R.; Sales, Susanne; Schuhmann, Kai; Schwartzman, Michal Laniado; Serhan, Charles N.; Shevchenko, Andrej; Somerville, Stephen E.; St. John-Williams, Lisa; Surma, Michal A.; Takeda, Hiroaki; Thakare, Rhishikesh; Thompson, J. Will; Torta, Federico; Triebl, Alexander; Trötzmüller, Martin; Ubhayasekera, S. J. Kumari; Vuckovic, Dajana; Weir, Jacquelyn M.; Welti, Ruth; Wenk, Markus R.; Wheelock, Craig E.; Yao, Libin; Yuan, Min; Zhao, Xueqing Heather; Zhou, Senlin
Journal of lipid research, 12/2017, Volume: 58, Issue: 12Journal Article
As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950–Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.
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