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Larsson, C.T. (Swedish Univ. of Agricultural Sciences, Uppsala (USA). Dept. of Cell Research); Khoshnoodi, J; Ek, B; Rask, L; Larsson, H
Plant molecular biology, (Jun 1998), 1998-Jun, 19980601, Volume: 37, Issue: 3Journal Article
Full-length cDNA for starch branching enzyme (SBE) II of potato was isolated and sequenced. In potato, similarly to most other investigated plants, the SBE-II isoform differs from SBE-I by having an acidic amino-terminal extension and a shorter carboxyterminus. Two forms of SBE-II, migrating as 98 and 95 kDa proteins in 6% SDS-polyacrylamide gels, were associated to tuber starch. The latter form was 16 amino acids shorter in the amino terminus. Transcript of SBE-II was present in leaf tissue, whereas significant expression was not seen in tubers. On the other hand, a significant amount of SBE-I transcript was detected in tuber tissue but not in leaves.
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