Excavations in the Roman villa of Aiano yielded twenty glass beads, a pendant, and a glass-recycling furnace, originally interpreted as a bead workshop. This article re-assesses the evidence of bead making in light of new data obtained thanks to recent progress in archaeological glass studies. A detailed study of the typology, technology, and chemical composition of the beads clearly excludes local production. Instead, two different forming techniques, four different base glasses (Roman, HIMT, Foy 2.1 and Foy 2.1/HIMT), and numerous colouring and opacifying materials point to a well-established and extensive network of the Roman bead trade, in which Aiano evidently participated. The majority of the beads can be related to the monumentalization of the villa in the fourth to fifth century ad and represent a sample of the ornaments worn by its inhabitants.