Leaf is an important organ for photosynthesis. Moderate leaf rolling could facilitate structure improvement of plant population and enhance light-use efficiency, which is important in breeding for ideotype plants. A rolled leaf mutant temporarily named rl12(t), was obtained from the rice ( Oryza sativa L.) restorer line Jinhui 10 treated with ethyl methyl sulphonate (EMS). In the mutant, the newly developing leaves of the mutant did not roll, the upper 1/3 section of mature leaves was curled, and the older mature leaves were rolled completely. The pigment contents of the mutant increased significantly. The cytoplasmic male sterile (CMS) line Xinong 1A with flat leaves was crossed with the rl12(t) mutant to produce F 1 and F 2 populations. Genetic analysis indicated that the mutant was controlled by a single dominant gene. Gene rl12(t) was finally located on chromosome 10 between SWU-1 and SWU-2 with the genetic distances of 1.5 and 0.2 cM, respectively. Because no genes for rolled leaf trait have been previously located on this chromosome, RL12(t) should be a novel and unique dominant gene for rolled leaf. 叶片是水稻光合作用的重要器官, 适度卷曲有利于改善群体光照、提高光能利用率, 卷叶基因是培育理想株型的重要资源。本研究利用EMS诱变优良恢复系缙恢10号, 获得了一个水稻新型卷叶突变体, 该性状受一对显性基因控制, 表现为新叶不卷, 老叶全卷, 而成熟叶片叶上部约1/3卷曲、中下部正常, 叶绿素含量极显著高于对照, 暂被命名为 rl12( t)。利用SSR标记将该基因定位于第10染色体SWU-1和SWU-2之间, 遗传距离分别是1.5 cM和0.2 cM。目前, 类似于 rl12( t)卷叶突变体表型未见报道, RL12( t)是唯一一个在第10染色体被分子定位的显性卷叶主基因。