Despite the significance of muscle oscillation frequency, previous research has not established a correlation with muscle performance due to the challenges of applying resistance without altering natural motion during functional tests. What is the correlation between muscle oscillation frequency and lower limb muscle strength, power, and work during an instrumented sit-to-stand and stand-to- sit (iSTS-TS) task among sedentary subjects? In a cross-sectional study, the oscillation frequency of the gastrocnemius medialis (GM), biceps femoralis (BF), and vastus medialis (VM) muscles in both the dominant (D) and non-dominant (ND) legs was assessed with a handheld myotonometer in 34 sedentary individuals before performing the iSTS-TS task. In the isokinetic mode, no significant correlations were found. In the isotonic mode, the BF muscle oscillation frequency in the D and ND legs exhibited significant positive correlations with peak force, peak power, and work during sitting down, as well as peak power and work during standing up. Positive correlations were observed in both legs between the GM oscillation frequency and sitting down peak force and work. Additionally, significant positive correlation was found with standing up work in the D leg. Muscle oscillation frequency of the VM exhibited a positive correlation with sitting down peak force in the ND leg. Due to a greater number of correlations found, it is advisable to use the isotonic mode when assessing muscle oscillation frequency in relation to muscle performance during functional iSTS-TS tasks in sedentary subjects. •Oscillation frequency correlates with limb performance in iSTS-TS task.•Isotonic mode reveals significant muscle performance correlations.•Assessment of iSTS-TS muscles in sedentary subjects via isotonic mode is recommended.