E-resources
-
Jin, Jung-Kang; Blackwood, Erik A; Azizi, Khalid; Thuerauf, Donna J; Fahem, Asal G; Hofmann, Christoph; Kaufman, Randal J; Doroudgar, Shirin; Glembotski, Christopher C
Circulation research, 03/2017, Volume: 120, Issue: 5Journal Article
RATIONALE:ER stress causes accumulation of misfolded proteins in the ER, activating the transcription factor, ATF6, which induces ER stress response genes. Myocardial ischemia induces the ER stress response; however, neither the function of this response nor whether it is mediated by ATF6 is known. OBJECTIVE:Here, we examined the effects of blocking the ATF6-mediated ER stress response on ischemia/reperfusion (I/R) in cardiac myocytes and mouse hearts. METHODS AND RESULTS:Knockdown of ATF6 in cardiac myocytes subjected to I/R increased ROS and necrotic cell death, which were mitigated by ATF6 overexpression. Under non-stressed conditions, WT and ATF6 knockout (KO) mouse hearts were similar. However, compared to WT, ATF6 KO hearts showed increased damage and decreased function upon I/R. Mechanistically, gene array analysis showed that ATF6, which is known to induce genes encoding ER proteins that augment ER protein-folding, induced numerous oxidative stress response genes not previously known to be ATF6-inducible. Many of the proteins encoded by the ATF6-induced oxidative stress genes identified here reside outside the ER, including catalase, which is known to decrease damaging ROS in the heart. Catalase was induced by the canonical ER stressor, tunicamycin, and by I/R in cardiac myocytes from WT but not in cardiac myocytes from ATF6 KO mice. ER stress response elements were identified in the catalase gene and were shown to bind ATF6 in cardiac myocytes, which increased catalase promoter activity. Overexpression of catalase, in vivo, restored ATF6 KO mouse heart function to WT levels in a mouse model of I/R, as did AAV9-mediated ATF6 overexpression. CONCLUSIONS:ATF6 serves as a previously unappreciated link between the ER stress and oxidative stress gene programs, supporting a novel mechanism by which ATF6 decreases myocardial I/R damage.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
If the library membership card is not in the list,
add a new one.
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|
Source: Personal bibliographies
and: SICRIS
The material is available in full text. If you wish to order the material anyway, click the Continue button.