The exploration of novel nonantibiotic interventions in the field, such as the use of bacteriophages, is necessary to avoid the presence of . Bacteriophages are a group of viruses widely distributed in nature, strictly associated with the prokaryotic cell. Researchers have demonstrated the success of phage therapy in reducing counts in poultry products. However, the impact that phage concentration in the environment may have against certain serovars is not well understood. Therefore, the aim of this study was to assess phage prevalence in commercial poultry farms in terms of the production type: layers or broilers. The most prevalent serovars isolated in poultry production were used for phage isolation. specific phages were isolated from 141 layer and broiler farms located in the Valencia region during 2019. Analysis of the samples revealed that 100% presented phages, the most prevalent being against serovar Enteritidis (93%), followed by Virchow (59%), Typhimurium (55%), Infantis (52%) and Ohio (51%). These results indicate that poultry farms could represent an important source of phages. Nevertheless, further studies are needed to assess the epidemiology of phages against other serovars present in other countries and their diversity from the point of view of molecular studies.