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Williamson, Laura; Saponaro, Marco; Boeing, Stefan; East, Philip; Mitter, Richard; Kantidakis, Theodoros; Kelly, Gavin P.; Lobley, Anna; Walker, Jane; Spencer-Dene, Bradley; Howell, Michael; Stewart, Aengus; Svejstrup, Jesper Q.
Cell, 02/2017, Volume: 168, Issue: 5Journal Article
The transcription-related DNA damage response was analyzed on a genome-wide scale with great spatial and temporal resolution. Upon UV irradiation, a slowdown of transcript elongation and restriction of gene activity to the promoter-proximal ∼25 kb is observed. This is associated with a shift from expression of long mRNAs to shorter isoforms, incorporating alternative last exons (ALEs) that are more proximal to the transcription start site. Notably, this includes a shift from a protein-coding ASCC3 mRNA to a shorter ALE isoform of which the RNA, rather than an encoded protein, is critical for the eventual recovery of transcription. The non-coding ASCC3 isoform counteracts the function of the protein-coding isoform, indicating crosstalk between them. Thus, the ASCC3 gene expresses both coding and non-coding transcript isoforms with opposite effects on transcription recovery after UV-induced DNA damage. Display omitted •UV elicits elongation slowdown and restricts transcription to the 5′ end of genes•UV induces a switch from long to short alternative last exon (ALE) transcript isoforms•ASCC3 short and long ALE isoforms have antagonistic functions in the UV response•The UV-induced ASCC3 short isoform functions as a long non-coding RNA UV damage generates a functional non-coding RNA through alternative pre-mRNA processing of a damage response factor transcript, identifying a pathway for repurposing protein coding genes under selective conditions.
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