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  • Toxic, cytotoxic and genoto...
    Batista, José Josenildo; de Araújo, Hallysson Douglas Andrade; Aguiar, Thierry Wesley de Albuquerque; Ferreira, Sílvio Assis de Oliveira; Lima, Maíra de Vasconcelos; Pereira, Dewson Rocha; Ferreira, Magda Rhayanny Assunção; Soares, Luiz Alberto Lira; Melo, Ana Maria Mendonça de Albuquerque; Albuquerque, Mônica Camelo Pessoa de Azevedo; Aires, André de Lima; Coelho, Luana Cassandra Breitenbach Barroso

    Acta tropica, April 2022, 2022-Apr, 2022-04-00, 20220401, Volume: 228
    Journal Article

    •The saline extract and the fraction (preparations) was toxic for embryonic stages of Biomphalaria glabrata.•The preparations showed toxicity for adult snails and cytotoxic and genotoxic effects on B. glabrata hemocytes.•The fraction revealed effects against Schistosoma mansoni cercariae and was not toxic to Artemia salina. This study describes for the first time the effect of saline extract and Parkia pendula seed fraction on Biomphalaria glabrata adult embryos and molluscs well as the reproductive parameters (fecundity and fertility) and survival, in addition to cytotoxicity and genotoxicity through the profile of blood cells after exposure to sublethal concentrations. Furthermore, we analyzed the action of both preparations against the cercariae of Schistosoma mansoni and their environmental safety using the bioindicator Artemia salina. The saline extract and fraction showed toxic effects for embryos (CL90 of 464.25, 479.62, 731.28, 643.28, 408.43 and 250.94, 318.03, 406.12, 635.64, 1.145 mg/mL, for blastula, gastrula, trocophore, veliger and hippo stage respectively), adult snails after 24 h of exposure (CL90 of 9.50 and 10.92 mg/mL, respectively) with increased mortality after 7 days of observation and significant decrease (p <0.05; p < 0.01 and p < 0.001) in egg mass deposition. At sublethal concentrations, an increase in quantitative and morphological changes in hemocytes was observed, and in the genotoxicity/comet assay analysis, varying degrees of nuclear damage were detected. In addition, the saline extract showed changes in the motility of the cercariae, while the fraction howed toxicity from a concentration of 1.0 mg/mL. The saline extract showed toxicity to A. salina at the highest concentrations (3.0, 4.0 and 5.0 mg/mL), while the fraction did not show ecotoxicity. Thus, the saline extract and fraction was promising in combating schistosomiasis by eliminating the intermediate host and causing alterations and/or mortality to the infectious agent. Display omitted