Physiological performance in lizards may be affected by climate across latitudinal or altitudinal gradients. In the coastal dune barriers in central‐eastern Argentina, the annual maximum environmental temperature decreases up to 2°C from low to high latitudes, while the mean relative humidity of the air decreases from 50% to 25%. Liolaemus multimaculatus, a lizard in the family Liolaemidae, is restricted to these coastal dunes. We investigated the locomotor performance of the species at 6 different sites distributed throughout its range in these dune barriers. We inquired whether locomotor performance metrics were sensitive to the thermal regime attributable to latitude. The thermal performance breadth increased from 7% to 82% with latitude, due to a decrease in its critical thermal minimum of up to 5°C at higher latitudes. Lizards from high latitude sites showed a thermal optimum, that is, the body temperature at which maximum speed is achieved, up to 4°C lower than that of lizards from the low latitude. At relatively low temperatures, the maximum running speed of high‐latitude individuals was faster than that of low‐latitude ones. Thermal parameters of locomotor performance were labile, decreasing as a function of latitude. These results show populations of L. multimaculatus adjust thermal physiology to cope with local climatic variations. This suggests that thermal sensitivity responds to the magnitude of latitudinal fluctuations in environmental temperature. The thermal performance curves of Liolaemus multimaculatus varied with latitude. The thermal optimum temperature for maximum running speed decreased with latitude. Lizards from high latitude showed high bouts of performance at a widest range of temperature. Locomotor performance metrics were sensitive to thermal regime attributable to latitude.