Abstract Introduction Infant sleep is influenced by biopsychosocial interactions. However, the influence of an interactive home environment is underexplored. The aim was to examine whether early positive parent-child interactions and an enriched home environment were associated with better infant sleep later. Methods Pregnant, obese, and low-income Latina women were recruited for a RCT delivering a 12-month intervention for infants to prevent overweight (n=129). At 1, 6, and 12 months, mothers reported breastfeeding duration, completed the Brief Infant Sleep Questionnaire-Revised (BISQ-R, range: 0-100), and underwent the Home Observation for Measurement of the Environment (HOME, range: 0-45) Inventory including subscales of Responsivity, Acceptance, Organization, Learning Materials, Involvement, and Variety. Pearson correlations between HOME and BISQ-R at each visit were conducted. Significant associations (α<0.05) were entered into stepwise multivariable linear regressions adjusting for infant sex, mother’s education, breastfeeding, and 1-month BISQ-R to determine which HOME subscales were associated with 6- and 12-month total BISQ-R if the total HOME was significant. Results The mean 1-, 6- and 12-month BISQ-R were 84(SD=6.1), 76.4(SD=8.3), and 78.5(SD=8.0), respectively. The 1-month HOME total score (M=21.8SD=4.4) significantly correlated with the 6- and 12-month BISQ-R. The regression of 1-month HOME with 6-month BISQ-R indicated greater BISQ-R scores were associated with shorter breastfeeding (β=-0.3, p=0.001), greater HOME total scores (β=0.2, p=0.037), and 1-month BISQ-R scores(β=0.2, p=0.038), which explained 13.1% of the variance (F3,106=6.5, p<0.001). The regression of 1-month HOME subscales with 6-month BISQ-R indicated greater BISQ-R scores were associated with shorter breastfeeding (β=-0.31, p=0.001), greater Responsivity scores (β=0.21, p=0.022), and 1-month BISQ-R scores (β=0.2, p=0.027), which explained 13.9% of the variance (F3,106=6.86, p<0.001). The regression of 1-month HOME with 12-month BISQ-R indicated greater BISQ-R scores were associated with shorter breastfeeding (β=-0.24, p=0.013) and greater HOME total scores (β=0.24, p=0.016), which explained 7.9% of the variance (F2,100=5.4, p=0.006). The regression of 1-month HOME subscales with 12-month BISQ-R indicated greater BISQ-R scores were associated with greater Variety scores (β=0.29, p=0.003), shorter breastfeeding (β=-0.24, p=0.011), and 1-month BISQ-R scores(β=0.18, p=0.049), which explained 13.5% of the variance (F3,99=6.29, p=0.001). Conclusion Better infant sleep was associated with an early interactive home environment, especially parent’s responsiveness and people/events providing organized variety. Support (if any):