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Muranli, Fulya Dilek Gökalp; Kanev, Martin; Ozdemir, Kezban
Arhiv za higijenu rada i toksikologiju, 6/2015, Volume: 66, Issue: 2Journal Article
The aim of this study was to evaluate the genetic damage in human peripheral blood lymphocytes following 24 and 48- hour exposure to a commercial diazinon formulation Basudin 60EM at concentrations between 0.01 and 40 μg mL . For this purpose we used the micronucleus (MN), fluorescence in situ hybridization (FISH), and alkaline single cell gel electrophoresis (comet) assay. Diazinon significantly increased the frequency of micronucleated cells compared to control. Forty-eight-hour exposure increased this frequency even at lower concentrations (0.01 μg mL ). The FISH results revealed aneugenic effects at 10 μg mL . The comet assay also confirmed DNA damage at concentrations between 10 and 40 μg mL . Our findings have confirmed the genotoxic potential of diazinon and its cytotoxic effect on human lymphocytes. The increased DNA damage in our study raises concern about the current assessment of the health risk posed by this pesticide and calls for a high level of caution in agricultural and household use. Cilj je ovog istraživanja bio ocijeniti genetička oštećenja u limfocitima ljudske periferne krvi nakon 24-satne odnosno 48-satne izloženosti komercijalnom diazinonu (Basudin 60EM ) u rasponu koncentracija od 0,01 do 40 μg mL . U tu smo svrhu rabili mikronukleus (MN)-test, fluorescencijsku in situ hibridizaciju (FISH) i metodu elektroforeze pojedinačnih stanica u agaroznom gelu (tzv. komet-test). Diazinon je značajno povećao učestalost stanica s mikronukleusima u odnosu na kontrolu. Taj je učinak bio još izraženiji nakon 48-satne izloženosti, gdje je značajno povećanje zamijećeno već pri koncentracijama diazinona od 0,01 do 10 μg mL . FISH je pokazao aneugeno djelovanje diazinona u koncentranciji od 10 μg mL . Naši rezultati potvrdili su ranija saznanja o genotoksičnom i citotoksičnom djelovanju diazonona na ljudske limfocite. Oštećenje DNA koje smo zamijetili u našem istraživanju dovodi u pitanje trenutačne procjene zdravstvenih rizika povezanih s tim pesticidom te poziva na izrazit oprez pri njegovoj primjeni u poljoprivredi i kućanstvima.
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