Drug-loaded electrospun nanofibers and films were fabricated in this study. The drug release of nanofibers was higher than that of films. The nanofibers possessed larger surface than films that drug could diffuse from the matrix easier. Additionally, amorphous state of drug in nanofibers and crystal in films made the different release behavior between nanofibers and films. Display omitted ▶ The release rate of FBF was found to increase with the increment of gelatin content. ▶ The FBF release rate of aligned nanofibrous scaffold was lower than that of randomly oriented scaffold. ▶ The crosslinking treatment decreased the burst release of FBF at initial release stage. ▶ The pH value of the buffer solution could affect the FBF release rate. Drug (Fenbufen, FBF)-loaded poly(d,l-lactide-co-glycolide) (PLGA) and PLGA/gelatin nanofibrous scaffolds were fabricated via electrospinning technique. The influences of gelatin content, fiber arrangement, crosslinking time and pH value of the buffer solution on FBF release behavior of the resulting nanofibrous scaffolds were investigated, with the corresponding FBF-loaded PLGA and PLGA/gelatin solvent-cast films as controls. The release rate of FBF was found to be increased with the increment of gelatin content for all the composite samples, and the FBF release rate of aligned nanofibrous scaffold was lower than that of randomly oriented scaffold. Moreover, the crosslinking treatment depressed effectively the burst release of FBF at initial release stage of PLGA/gelatin (9/1) nanofibrous scaffold. In addition, the pH value of the buffer solution could change the physical state of the polymer and affect the FBF release rate.