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Vlachogiannis, Nikolaos I.; Sachse, Marco; Georgiopoulos, Georgios; Zormpas, Eleftherios; Bampatsias, Dimitrios; Delialis, Dimitrios; Bonini, Francesca; Galyfos, George; Sigala, Fragiska; Stamatelopoulos, Kimon; Gatsiou, Aikaterini; Stellos, Konstantinos
Journal of molecular and cellular cardiology, November 2021, 2021-11-00, 20211101, Volume: 160Journal Article
Long non-coding RNAs (lncRNAs) have emerged as critical regulators in human disease including atherosclerosis. However, the mechanisms involved in the post-transcriptional regulation of the expression of disease-associated lncRNAs are not fully understood. Gene expression studies revealed that Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) lncRNA expression was increased by >2-fold in peripheral blood mononuclear cells (PBMCs) derived from patients with coronary artery disease (CAD) or in carotid artery atherosclerotic plaques. We observed a linear association between NEAT1 lncRNA expression and prevalence of CAD which was independent of age, sex, cardiovascular traditional risk factors and renal function. NEAT1 expression was induced by TNF-α, while silencing of NEAT1 profoundly attenuated the TNF-α-induced vascular endothelial cell pro-inflammatory response as defined by the expression of CXCL8, CCL2, VCAM1 and ICAM1. Overexpression of the RNA editing enzyme adenosine deaminase acting on RNA-1 (ADAR1), but not of its editing-deficient mutant, upregulated NEAT1 levels. Conversely, silencing of ADAR1 suppressed the basal levels and the TNF-α-induced increase of NEAT1. NEAT1 lncRNA expression was strongly associated with ADAR1 in CAD and peripheral arterial vascular disease. RNA editing mapping studies revealed the presence of several inosines in close proximity to AU-rich elements within the AluSx3+/AluJo‐ double-stranded RNA complex. Silencing of the stabilizing RNA-binding protein AUF1 reduced NEAT1 levels while silencing of ADAR1 profoundly affected the binding capacity of AUF1 to NEAT1. Together, our findings propose a mechanism by which ADAR1-catalyzed A-to-I RNA editing controls NEAT1 lncRNA stability in ASCVD. Display omitted •Expression of NEAT1 lncRNA is increased in human coronary artery disease (CAD) and in carotid artery atherosclerotic plaques.•NEAT1 lncRNA controls endothelial cell innate immune response to TNF-α.•Alu RNA editing events on NEAT1 lncRNA are located within close proximity to AUF1 binding sites.•ADAR1 controls NEAT1 stability by enabling the recruitment of AUF1 to NEAT1 lncRNA.
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